Using the CL-770 Spectrophotometer to Quantify Serum Immunoglobulins

Serum immunoglobulins (A, G, M) are usually quantified by radial gel immunodiffusion (RID). It is based on measuring the diameter of the precipitation ring formed when the serum is added to the wells cut out in the agar layer in which the monospecific antiserum is preliminarily dispersed. The gel plates are placed in a humid chamber for 24-48 hours. At the end of the incubation, the diameter of the precipitation ring is measured. Under standard experimental conditions, the diameter of the precipitation ring is directly proportional to the concentration of the studied immunoglobulin. The amount of immunoglobulins is determined by constructing a calibration graph. The results of determining the concentration of serum immunoglobulins by the RID method are issued only on the 3rd day of the study. Clinicians are not always satisfied with the timing of the results and the quality of the study, since the latter depends on many factors - thoroughness (conducting the experiment, the quality and thickness of the gel, the scale of the schedule, etc.)