Hematological changes and structural morphological changes in the liver and spleen in mice against the background of acute toxic effects of cyclophosphan
- Authors: Kokaya G.N.1,2, Kokaya A.A.1, Vasilieva E.A.3,4, Guseva M.S.3, Mukhina I.V.3
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Affiliations:
- Aviastankoservice LLC
- National Medical Research Center named after V.A. Almazov
- Privolzhsky Research Medical University
- Institute of Biology and Biomedicine, National Research Nizhny Novgorod State University named after N.I. Lobachevsky
- Issue: Vol 104, No 4 (2023)
- Pages: 523-533
- Section: Experimental medicine
- URL: https://kazanmedjournal.ru/kazanmedj/article/view/321903
- DOI: https://doi.org/10.17816/KMJ321903
- ID: 321903
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Abstract
Background. Disturbances in the body associated with bone marrow failure caused by the administration of lethal doses of cyclophosphan to animals make it possible to use this experimental model to solve important pathophysiological problems.
Aim. To reveal hematological changes and structural morphological changes in the liver and spleen in mice against the background of acute toxic effects of lethal doses of.cyclophosphan.
Material and methods. The studies were performed on 200 outbred male mice weighing 27–29 g. Acute cytotoxic syndrome was modeled by a single intraperitoneal injection of a solution of cyclophosphan at doses of 500 and 750 mg/kg of body weight. Blood sampling for hematological studies was carried out on the 3rd, 6th, 10th and 15th days of intoxication, and the removal of the liver and spleen for morphological studies was performed on the 6th and 15th days. Statistical analysis was performed using Statistica 10 and MS Excel. Results were presented as mean (M) ± standard error of the mean (mx). To determine the significance of differences between groups with a significance level of p <0.05, Fisher's test and Mann–Whitney U-test were used.
Results. Mortality against the background of acute cytotoxic action of cyclophosphan at doses of 500 and 750 mg/kg reached 83.3 and 100%. The initial content of leukocytes in the peripheral blood of mice was (8.6±2.3)×109/l, and on the 6th day of intoxication it decreased to (1.0±0.3)×109/l and (0.6±0.1)×109/l (p=0.01). Blast cells of all hematopoietic lineages were present in blood smears. In the morphological picture of the spleen, the connective tissue stroma was sharply distinguished, and the white and red pulps were almost completely absent. Signs of acute toxic effects of cyclophosphan were found in the liver tissue.
Conclusion. The course of acute cytotoxic syndrome caused by cyclophosphan is accompanied by high mortality, the development of pancytopenia, pronounced destructive changes in the spleen tissue and toxic changes in the liver tissue.
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About the authors
Georgiy Nikolaevich Kokaya
Aviastankoservice LLC; National Medical Research Center named after V.A. Almazov
Email: kkgeo@yandex.ru
ORCID iD: 0009-0003-6482-0171
Junior Researcher; Clinical Resident, Depart. of Faculty Therapy
Russian Federation, Moscow, Russia; St. Petersburg, RussiaAnna A. Kokaya
Aviastankoservice LLC
Author for correspondence.
Email: kann1812@yandex.ru
ORCID iD: 0009-0008-9659-5315
M.D., Cand. Sci. (Med.), Senior Researcher
Russian Federation, Moscow, RussiaEkaterina Aleksandrovna Vasilieva
Privolzhsky Research Medical University; Institute of Biology and Biomedicine, National Research Nizhny Novgorod State University named after N.I. Lobachevsky
Email: VasilyevaKatya@yandex.ru
ORCID iD: 0000-0002-5559-282X
Cand. Sci. (Biol.), Assoc. Prof., Depart. of Normal Physiology named after N.Y. Belenkov; Research Associate, Central Research Laboratory; Department of Biochemistry and Biotechnology, Institute of Biology and Biomedicine
Russian Federation, Nizhny Novgorod, Russia; Nizhny Novgorod, RussiaMaria S. Guseva
Privolzhsky Research Medical University
Email: gusevamaria1@gmail.com
ORCID iD: 0000-0003-1493-8000
Junior Researcher, Depart. of Morphology, Central Research Laboratory
Russian Federation, Nizhny Novgorod, RussiaIrina V. Mukhina
Privolzhsky Research Medical University
Email: mukchinaiv@mail.ru
ORCID iD: 0000-0002-8811-0049
D. Sci. (Biol.), Head of Depart., Depart. of Normal Physiology named after N.Y. Belenkov
Russian Federation, Nizhny Novgorod, RussiaReferences
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