Levels of biogenic amines in the brain of rats at experimental post-traumatic stress disorder development
- Authors: Deev RV1, Shatrova Y.M1, Sinitskiy AI1, Molchanova NS1, Yunusova AK1, Tseylikman OB2, Kozochkin DA1, Lapshin MS2
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Affiliations:
- South Ural State Medical University, Chelyabinsk, Russia
- National Research South Ural State University, Chelyabinsk, Russia
- Issue: Vol 96, No 5 (2015)
- Pages: 806-810
- Section: Experimental medicine
- URL: https://kazanmedjournal.ru/kazanmedj/article/view/2032
- DOI: https://doi.org/10.17750/KMJ2015-806
- ID: 2032
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Full Text
Abstract
Aim. To study the changes in levels of biogenic amines-neurotransmitters in the brain at experimental post-traumatic stress disorder development in rats.
Methods. Post-traumatic stress disorder was modeled by keeping 48 outbred male rats in under constant and inescapable strong unconditioned stimulus. The control group included 16 intact animals, not exposed to stress influences. The levels of 3,4-dihydroxyphenylalanine, dopamine, norepinephrine, epinephrine and gamma-aminobutyric acid were determined by fluorometric methods. Behavioral activity of animals was evaluated on the day 3, 7, 10 and 14 by «open field» and «elevated plus maze» actinographs.
Results. When comparing the concentrations of studied neurotransmitters in the brain of control animals with experimental groups, reflecting the development of post-traumatic stress disorder at the time, adrenaline and 3,4-dihydroxyphenylalanine levels were increased on the third day, level of norepinephrine was reduced on the seventh day, 3,4-dihydroxyphenylalanine, dopamine, norepinephrine levels were elevaled, gamma-aminobutyric acid level was reduced on the tenth day, gamma-aminobutyric acid level was increased on the fourteenth day after the stress.
Conclusion. According to the results of the correlation analysis, the largest contribution to the development of behavioral disorders are made by altered brain level of gamma-aminobutyric acid at the time of post-traumatic stress disorder formation (tenth and fourteenth day). At the earlier stages (third and seventh day), the relationship of rats behavioral activity and altered 3,4-dihydroxyphenylalanine and norepinephrine brain levels was shown.
Keywords
About the authors
R V Deev
South Ural State Medical University, Chelyabinsk, Russia
Author for correspondence.
Email: Sinitskiyai@yandex.ru
Yu M Shatrova
South Ural State Medical University, Chelyabinsk, Russia
Email: Sinitskiyai@yandex.ru
A I Sinitskiy
South Ural State Medical University, Chelyabinsk, Russia
Email: Sinitskiyai@yandex.ru
N S Molchanova
South Ural State Medical University, Chelyabinsk, Russia
Email: Sinitskiyai@yandex.ru
A K Yunusova
South Ural State Medical University, Chelyabinsk, Russia
Email: Sinitskiyai@yandex.ru
O B Tseylikman
National Research South Ural State University, Chelyabinsk, Russia
Email: Sinitskiyai@yandex.ru
D A Kozochkin
South Ural State Medical University, Chelyabinsk, Russia
Email: Sinitskiyai@yandex.ru
M S Lapshin
National Research South Ural State University, Chelyabinsk, Russia
Email: Sinitskiyai@yandex.ru
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