Kazan medical journalKazan medical journal0368-48142587-9359Eco-Vector203210.17750/KMJ2015-806Research ArticleLevels of biogenic amines in the brain of rats at experimental post-traumatic stress disorder developmentDeevR VSinitskiyai@yandex.ruShatrovaYu MSinitskiyai@yandex.ruSinitskiyA ISinitskiyai@yandex.ruMolchanovaN SSinitskiyai@yandex.ruYunusovaA KSinitskiyai@yandex.ruTseylikmanO BSinitskiyai@yandex.ruKozochkinD ASinitskiyai@yandex.ruLapshinM SSinitskiyai@yandex.ruSouth Ural State Medical University, Chelyabinsk, RussiaNational Research South Ural State University, Chelyabinsk, Russia1510201596580681028032016Copyright © 2015, Deev R.V., Shatrova Y.M., Sinitskiy A.I., Molchanova N.S., Yunusova A.K., Tseylikman O.B., Kozochkin D.A., Lapshin M.S.2015<p><strong>Aim.</strong> To study the changes in levels of biogenic amines-neurotransmitters in the brain at experimental post-traumatic stress disorder development in rats.</p>
<p><strong>Methods.</strong> Post-traumatic stress disorder was modeled by keeping 48 outbred male rats in under constant and inescapable strong unconditioned stimulus. The control group included 16 intact animals, not exposed to stress influences. The levels of 3,4-dihydroxyphenylalanine, dopamine, norepinephrine, epinephrine and gamma-aminobutyric acid were determined by fluorometric methods. Behavioral activity of animals was evaluated on the day 3, 7, 10 and 14 by «open field» and «elevated plus maze» actinographs.</p>
<p><strong>Results.</strong> When comparing the concentrations of studied neurotransmitters in the brain of control animals with experimental groups, reflecting the development of post-traumatic stress disorder at the time, adrenaline and 3,4-dihydroxyphenylalanine levels were increased on the third day, level of norepinephrine was reduced on the seventh day, 3,4-dihydroxyphenylalanine, dopamine, norepinephrine levels were elevaled, gamma-aminobutyric acid level was reduced on the tenth day, gamma-aminobutyric acid level was increased on the fourteenth day after the stress.</p>
<p><strong>Conclusion.</strong> According to the results of the correlation analysis, the largest contribution to the development of behavioral disorders are made by altered brain level of gamma-aminobutyric acid at the time of post-traumatic stress disorder formation (tenth and fourteenth day). At the earlier stages (third and seventh day), the relationship of rats behavioral activity and altered 3,4-dihydroxyphenylalanine and norepinephrine brain levels was shown.</p>post-traumatic stress disorderneurotransmittersbrainпосттравматическое стрессовое расстройствонейромедиаторыголовной мозг[Бузыкина Ю.С., Константинов В.В. Изучение смысложизненных ориентаций и уровня переживания террористической угрозы у представителей трёх поколений // Психол. и право. - 2013. - №1. - С. 33-43.][Камышников В.С. Справочник по клинико-биохимическим исследованиям и лабораторной диагностике. - М.: МЕДпресс-информ, 2009. - 896 с.][Кутлубаев А.А., Ахмадеева Л.Р. Стрессовое расстройство после инсульта: частота, факторы риска, подходы к профилактике и лечению // Неврол., нейропсихиатр., психосомат. - 2014. - №2. - С. 70-74.][Тарабрина Н.В. Практикум по психологии посттравматического стресса. - СПб.: Питер, 2001. - 272 с.][Тарабрина Н.В., Курчакова М.С. Психофизиологические корреляты посттравматического стресса у онкологических больных // Социал. и клин. психиатрия. - 2007. - Т. 17, №4. - С. 17-23.][Тарабрина Н.В., Быховец Ю.В., Ворона О.А. Проблема психологического благополучия населения перед лицом террористической угрозы // Учёные записки забайкал. гос. ун-та. Сер.: Философия, социол., культурол., социал. работа. - 2009. - №4. - С. 61-68.][Avedisova A.S. Psychopharmacotherapy of patients with posttraumatic stress disorder // Zh. Nevrol. Psikhiatr. Im. S.S. Korsakova. - 2009. - Vol. 109, N 12. - P. 46-49.][Bellis M.D., Baum A.S., Birmaher B., Ryan N.D. Urinary catecholamine excretion in childhood overanxious and posttraumatic stress disorders // Ann. NY Acad. Sci. - 1997. - Vol. 821, N 1. - P. 451-455. http://dx.doi.org/10.1111/j.1749-6632.1997.tb48303.x][Cohen H., Matar M.A., Joseph Z. Animal models of post-traumatic stress disorder // Curr. Protocols in Neurosci. - 2013. - Vol. 7, N 9. - P. 9.45.1-9.45.18. http://dx.doi.org/10.1002/0471142301.ns0945s64][Geracioti T.D.Jr, Baker D.G., Ekhator N.N. et al. CSF norepinephrine concentrations in posttraumatic stress disorder // Am. J. Psychiatry. - 2001. - Vol. 158, N 8. - P. 1227-1230. http://dx.doi.org/10.1176/appi.ajp.158.8.1227][Jeffreys M., Capehart B., Friedman M.J. Pharmacotherapy for posttraumatic stress disorder: review with clinical applications // J. Rehabil. Res. Dev. - 2012. - Vol. 49, N 5. - P. 703-715. http://dx.doi.org/10.1682/JRRD.2011.09.0183][Michels L., Schulte-Vels T., Schick M. et al. Prefrontal GABA and glutathione imbalance in posttraumatic stress disorder: Preliminary findings // Psychiatry Res. Neuroimaging. - 2014. - Vol. 224, N 3. - P. 288-295. http://dx.doi.org/10.1016/j.pscychresns.2014.09.007][Sutton I., Simmonds M.A. Effects of acute and chronic pentobarbitone on the γ-aminobutyric acid system in rat brain // Biochem. Pharm. - 1974. - Vol. 23, N 13. - P. 1801-1808. http://dx.doi.org/10.1016/0006-2952(74)90188-9]