Effect of benzimidazole derivative containing dioxonium ethane cycle on the outcomes of emotional stress induced by physical overload in animals

Aim. To study the outcomes of emotional stress induced by physical overload, and to assess the effects of benzimidazole derivative containing dioxonium ethane cycle of 2-[1-(1,1-dioxyethenyl 3) benzimidazolyl-2-thio] acetic acid potassium salt.

Methods. Behavioral responses were studied in experimental animals getting graduated physical exercise (swimming test) which was used as a model for physical and emotional stress. White scrub rats with a body weight of 120-180 g were used as research object. Physical overload was induced by making animals swim 30 minutes daily for 28 days. Animals were distributed into three groups of 10 animals each: the control group, the group of physical activity, and the group of physical activity exposed to the studied substance (50 mg/kg).

Results. Animals exposed to physical activity showed an increase in adrenal glands weight white blood cell count and reduced the mobility ratio and the overall research activity, as well as increased emotional anxiety, indicating stress. Benzimidazole derivative showed to be effective controller of chronic stress. Physical activity in animals exposed to study substance did not lead to an increase of white blood cell count and adrenal glands weight. Animals exposed to study substance increased mobility ratio and the overall research activity by 1.5 times, and the rate of emotional anxiety in animals has decreased by 2 times compared to the group that was exposed to physical activity without benzimidazole derivative administration. Free radical oxidation processes were more intense in brain homogenates of animals after physical activity, which was seen as increased levels of reactants interacting with thiobarbituric acid and decreased total antioxidant activity. In animals exposed to study substance, these indicators increased compared to animals exposed physical activity without administration of study substance, and were comparable to controls.

Conclusion. The use of the study substance prevents the negative effects of emotional stress and physical overload.