Association of p53 protein expression with the presence of a 17p13 locus deletion of the TP53 gene and with the survival of patients with diffuse large B-cell lymphoma
- Authors: Sarpova M.V.1, Vaneeva E.V.1, Diakonov D.A.1, Rosin V.A.1, Samarina S.V.1
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Affiliations:
- Kirov Research Institute of Hematology and Blood Transfusion of the Federal Medical and Biological Agency
- Issue: Vol 104, No 1 (2023)
- Pages: 38-46
- Section: Theoretical and clinical medicine
- Submitted: 01.04.2022
- Accepted: 03.11.2022
- Published: 01.02.2023
- URL: https://kazanmedjournal.ru/kazanmedj/article/view/105310
- DOI: https://doi.org/10.17816/KMJ105310
- ID: 105310
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Abstract
Background. A significant role in the pathogenesis, resistance to treatment and progression of many types of lymphomas, including diffuse large B-cell lymphoma, is assigned to the TP53 gene. Literature data on the prognostic significance of the expression of its product, the p53 protein, and its association with aberrations at the 17p13 locus are ambiguous.
Aim. To assess the relationship of p53 protein expression with the presence of a 17p13 locus deletion of the TP53 gene and the survival of patients with diffuse large B-cell lymphoma.
Material and methods. The study included 75 patients with newly diagnosed diffuse large B-cell lymphoma who received R-CHOP therapy. The calculation of the relative content of tumor cells expressing p53 was carried out using immunohistochemical and morphometric methods on biopsy samples of lymph nodes. The 17p13/TP53 deletion was determined by fluorescent in situ hybridization. The threshold level of p53-positive tumor cells, corresponding to 43%, was calculated by ROC analysis. The association between p53 protein expression and the presence of a 17p13 deletion was assessed using Fisher's (F) and Pearson's χ2 tests. The correlation dependence was evaluated by the Cramer method (V). Five-year overall and progression-free survival was calculated using the Kaplan–Meier method. Differences between the indicators were considered statistically significant at p <0.05.
Results. The frequency of 17p13/TP53 deletion was higher in patients with high expression of p53 (≥43%) compared to the group of patients with low expression: 87.5 and 12.5%, respectively (p=0.018). A direct correlation between a high level of p53 expression (>43%) and the presence of a 17p13/TP53 deletion was found (p=0.018). Five-year overall and progression-free survival in patients with a proportion of p53-positive cells> 43% was significantly lower than in patients with its subthreshold value: 54.5 versus 81.0% (p=0.014) and 45.5 versus 66.7% (p=0.022), respectively.
Conclusion. High expression of the p53 protein is associated with the presence of a 17p13 locus deletion and a low five-year survival rate in patients with diffuse large B-cell lymphoma.
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About the authors
Mariia V. Sarpova
Kirov Research Institute of Hematology and Blood Transfusion of the Federal Medical and Biological Agency
Author for correspondence.
Email: marisarpova@mail.ru
ORCID iD: 0000-0001-5949-7865
SPIN-code: 8444-6740
Researcher, Laboratory of Pathomorphology
Russian Federation, Kirov, RussiaElena V. Vaneeva
Kirov Research Institute of Hematology and Blood Transfusion of the Federal Medical and Biological Agency
Email: vaneeva@niigpk.ru
ORCID iD: 0000-0003-1045-2011
SPIN-code: 9663-3399
Scopus Author ID: 57222653084
Researcher, Laboratory of Pathomorphology
Russian Federation, Kirov, RussiaDmitry A. Diakonov
Kirov Research Institute of Hematology and Blood Transfusion of the Federal Medical and Biological Agency
Email: dyakonov@niigpk.ru
ORCID iD: 0000-0001-8688-1344
SPIN-code: 6301-6557
Scopus Author ID: 55920048300
M.D., Cand. Sci. (Med.), Head, Laboratory of Pathomorphology
Russian Federation, Kirov, RussiaVitaly A. Rosin
Kirov Research Institute of Hematology and Blood Transfusion of the Federal Medical and Biological Agency
Email: rosin@niigpk.ru
ORCID iD: 0000-0003-2054-2870
SPIN-code: 8058-3942
Scopus Author ID: 55893734700
M.D., Cand. Sci. (Med.), Senior Researcher, Laboratory of Pathomorphology
Russian Federation, Kirov, RussiaSvetlana V. Samarina
Kirov Research Institute of Hematology and Blood Transfusion of the Federal Medical and Biological Agency
Email: samarina@niigpk.ru
ORCID iD: 0000-0001-8639-719X
M.D., Head, Clinical Diagnostic Depart.
Russian Federation, Kirov, RussiaReferences
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