Characteristics of immunocompetent cells in the testicles of different age group patients with COVID-19

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Abstract

BACKGROUND: There is an opinion that the new coronavirus infection COVID-19 can initiate the development of a local inflammatory reaction in the testicles, which leads to damage to spermatogenic epithelial cells and a decrease in the fertility of patients in the long term.

AIM: Morphofunctional assessment of immunocompetent cells in the testicles of patients with COVID-19 depending on age.

MATERIAL AND METHODS: Based on anamnestic, clinical and morphological data, groups of patients were formed, each of which included subgroups according to the age periodization of the World Health Organization: the first group of patients who died as a result of COVID-19 (n=109; average age 58±2.8 years) — young subgroup (n=19, age 18–44 years), middle-aged subgroup (n=37, age 45–59 years), elderly subgroup (n=53, age 60–74 years); second group (n=30, average age 49±2.3 years; autopsy material from the testicles of patients who died from causes unrelated to COVID-19, obtained outside the pandemic) — a subgroup of young people (n=10, age 18–44 years), middle-aged subgroup (n=10, age 45–59 years), elderly subgroup (n=10, age 60–74 years). Histological and immunohistochemical studies were carried out using primary antibodies to CD3, CD4, CD68, CD163, CD138 and statistical methods: Kolmogorov–Smirnov test, Student's t-test, Mann–Whitney U test and Fisher test.

RESULTS: All testicular samples from patients with COVID-19 revealed signs of viral orchitis and a significant decrease in the spermatogenesis index (in young people — 5.9±0.2% with p=0.02; in middle age — 5.1±0.2% with p=0.008; in the elderly — 3.6±0.1% at p=0.006) compared to the control group (6.8±0.3%), and in immunohistochemical studies — an increase in the number of T-lymphocytes (CD3+, CD4+), macrophages (CD68+, CD163+) and plasma cells (CD138+) in interstitial tissue. In addition, a significant decrease in the spermatogenesis index (3.6±0.1% in the elderly versus 5.9±0.2% in the young, p=0.007) and a decrease in the number of T-lymphocytes (CD3+, CD4+) and macrophages (CD68+, CD163+) was found in older versus younger patients with COVID-19.

CONCLUSION: In patients with COVID-19, an increase in the number of immunocompetent cells (CD3+, CD4+, CD68+, CD138+, CD163+) in the interstitial tissue of the testicles was found, especially pronounced in the elderly group.

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About the authors

Grigory A. Demyashkin

First Moscow State Medical University named after I.M. Sechenov (Sechenov University); National Medical Research Center for Radiology

Author for correspondence.
Email: dr.dga@mail.ru
ORCID iD: 0000-0001-8447-2600
SPIN-code: 5157-0177

MD, Dr. Sci. (Med.), Head of the Laboratory, Laboratory of Histology and Immunohistochemistry, Institute of Translational Medicine and Biotechnology

Russian Federation, Moscow; Moscow

Dmitry V. Boldyrev

First Moscow State Medical University named after I.M. Sechenov (Sechenov University)

Email: Derfeelgood@yandex.ru
ORCID iD: 0000-0002-4548-5430
SPIN-code: 1893-4960

MD, Pathologist, Post-Graduate Student, Institute of Translational Medicine and Biotechnology

Russian Federation, Moscow

Matvey A. Vadyukhin

First Moscow State Medical University named after I.M. Sechenov (Sechenov University)

Email: vma20@mail.ru
ORCID iD: 0000-0002-6235-1020
SPIN-code: 9485-7722

Stud., N.V. Sklifosovsky Institute of Clinical Medicine

Russian Federation, Moscow

Lia N. Alieva

First Moscow State Medical University named after I.M. Sechenov (Sechenov University)

Email: pancake0401@gmail.com
ORCID iD: 0009-0002-9989-7868

Stud., N.V. Sklifosovsky Institute of Clinical Medicine

Russian Federation, Moscow

Elmar N. Shirinov

First Moscow State Medical University named after I.M. Sechenov (Sechenov University)

Email: ekaqaqa@mail.ru
ORCID iD: 0009-0000-9799-8601

Stud., Institute of Dentistry of the Sechenov University

Russian Federation, Moscow

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Supplementary files

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2. Fig. 1. Morphological picture of the testicle of a patient with confirmed new coronavirus infection. Hematoxylin and eosin staining: a — magnification ×200; b — magnification ×400

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3. Fig. 2. Immunohistochemical study of the testicles of patients with COVID-19, staining with hematoxylin, magnification ×400

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