Neuropeptide Y is involved in the regulation of the frequency and strength of rat atrium spontaneous contraction
- Authors: Iskakov N.G.1,2, Anikina T.A.1, Zefirov A.L.3, Bilalova G.A.1, Zefirov T.L.1
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Affiliations:
- Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University
- Volga Region State University of Physical Culture, Sports and Tourism
- Kazan State Medical University
- Section: Original research
- Submitted: 26.06.2023
- Accepted: 25.06.2024
- Published: 08.07.2024
- URL: https://kazanmedjournal.ru/kazanmedj/article/view/501790
- DOI: https://doi.org/10.17816/KMJ501790
- ID: 501790
Cite item
Abstract
BACKGROUND: The relevance of the problem increases due to the established cardioprotective effect of neuropeptide Y in pathological conditions such as hypertension, heart failure, and its possible therapeutic use in cardiovascular diseases.
AIM: Study of the effect of neuropeptide Y on the frequency of spontaneous activity and amplitude-time parameters of the myocardium contraction of the rats’ right atrium during different periods of postnatal ontogenesis.
MATERIAL AND METHODS: The experiments used 73 white outbred rats of 7 days (n=23), 21 days (n=25) and 100 days (n=25) of age. Using the classical strain-gauge method, the parameters of isometric contraction of isolated rat atrial myocardium were determined with the addition of a non-selective Y-receptor agonist. The isometric contraction curve was processed using the Chart 8.0 program. Statistical processing of the results was performed in MS Excel and IBM SPSS Statistics 2020. The significance of the differences was calculated using the Student’s t test. Data were presented as mean ± error of mean (M±m); n was the number of atrial myocardium preparations used. Changes were considered statistically significant at p <0.05.
RESULTS: Neuropeptide Y at a concentration of 10–7 M reduces the frequency of spontaneous activity of the right atrium strips of rats with a preserved sinus node in 7, 21 and 100-day-old animals by 8.1%, 15% (p=0.038) and 7.9% (p=0.046) respectively in comparison with the control value. The amplitude of isometric contraction of the atrial myocardium in 7-day-old animals decreases by 12.4% (p=0.041), the duration of contraction decreases in 21-day-old animals by 12% (p=0.040), and in 100-day-old animals does not change compared to control.
CONCLUSION: Neuropeptide Y reduces the frequency of spontaneous activity, increases the contraction duration, reduces the isometric contraction amplitude of rat myocardial strips in early postnatal ontogenesis.
Keywords
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About the authors
Nikita G. Iskakov
Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University; Volga Region State University of Physical Culture, Sports and Tourism
Author for correspondence.
Email: nikitaiskakov1992@mail.ru
ORCID iD: 0000-0002-3737-9363
SPIN-code: 4784-4856
Cand. Sci. (Biol.), Senior Lecturer, Depart. of Human Health Protection; Assoc. Prof., Depart. of Biomedical Disciplines
Russian Federation, Kazan; KazanTatyana A. Anikina
Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University
Email: tania57vg1@rambler.ru
ORCID iD: 0000-0002-2020-3054
SPIN-code: 8076-6386
Dr. Sci. (Biol.), Prof.
Russian Federation, KazanAndrey L. Zefirov
Kazan State Medical University
Email: zefirov@kmu-mf.kcn.ru
ORCID iD: 0000-0001-7436-7815
SPIN-code: 6239-1965
MD, Academician of the Russian Academy of Sciences, Dr. Sci. (Med.), Prof.
Russian Federation, KazanGulfiya A. Bilalova
Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University
Email: g.bilalova@mail.ru
ORCID iD: 0000-0002-2534-5027
SPIN-code: 5947-3667
Cand. Sci. (Biol.), Assoc. Prof., Depart. of Human Health
Russian Federation, KazanTimur L. Zefirov
Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University
Email: zefirovtl@mail.ru
ORCID iD: 0000-0001-9557-1639
SPIN-code: 5267-1350
Dr. Sci. (Biol.), Prof., Head of Depart., Depart. of Human Health Protection
Russian Federation, KazanReferences
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