Neuropeptide Y is involved in the regulation of the frequency and strength of rat atrium spontaneous contraction



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Abstract

BACKGROUND: The relevance of the problem increases due to the established cardioprotective effect of neuropeptide Y in pathological conditions such as hypertension, heart failure, and its possible therapeutic use in cardiovascular diseases.

AIM: Study of the effect of neuropeptide Y on the frequency of spontaneous activity and amplitude-time parameters of the myocardium contraction of the rats’ right atrium during different periods of postnatal ontogenesis.

MATERIAL AND METHODS: The experiments used 73 white outbred rats of 7 days (n=23), 21 days (n=25) and 100 days (n=25) of age. Using the classical strain-gauge method, the parameters of isometric contraction of isolated rat atrial myocardium were determined with the addition of a non-selective Y-receptor agonist. The isometric contraction curve was processed using the Chart 8.0 program. Statistical processing of the results was performed in MS Excel and IBM SPSS Statistics 2020. The significance of the differences was calculated using the Student’s t test. Data were presented as mean ± error of mean (M±m); n was the number of atrial myocardium preparations used. Changes were considered statistically significant at p <0.05.

RESULTS: Neuropeptide Y at a concentration of 10–7 M reduces the frequency of spontaneous activity of the right atrium strips of rats with a preserved sinus node in 7, 21 and 100-day-old animals by 8.1%, 15% (p=0.038) and 7.9% (p=0.046) respectively in comparison with the control value. The amplitude of isometric contraction of the atrial myocardium in 7-day-old animals decreases by 12.4% (p=0.041), the duration of contraction decreases in 21-day-old animals by 12% (p=0.040), and in 100-day-old animals does not change compared to control.

CONCLUSION: Neuropeptide Y reduces the frequency of spontaneous activity, increases the contraction duration, reduces the isometric contraction amplitude of rat myocardial strips in early postnatal ontogenesis.

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About the authors

Nikita G. Iskakov

Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University; Volga Region State University of Physical Culture, Sports and Tourism

Author for correspondence.
Email: nikitaiskakov1992@mail.ru
ORCID iD: 0000-0002-3737-9363
SPIN-code: 4784-4856

Cand. Sci. (Biol.), Senior Lecturer, Depart. of Human Health Protection; Assoc. Prof., Depart. of Biomedical Disciplines

Russian Federation, Kazan; Kazan

Tatyana A. Anikina

Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University

Email: tania57vg1@rambler.ru
ORCID iD: 0000-0002-2020-3054
SPIN-code: 8076-6386

Dr. Sci. (Biol.), Prof.

Russian Federation, Kazan

Andrey L. Zefirov

Kazan State Medical University

Email: zefirov@kmu-mf.kcn.ru
ORCID iD: 0000-0001-7436-7815
SPIN-code: 6239-1965

MD, Academician of the Russian Academy of Sciences, Dr. Sci. (Med.), Prof.

Russian Federation, Kazan

Gulfiya A. Bilalova

Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University

Email: g.bilalova@mail.ru
ORCID iD: 0000-0002-2534-5027
SPIN-code: 5947-3667

Cand. Sci. (Biol.), Assoc. Prof., Depart. of Human Health

Russian Federation, Kazan

Timur L. Zefirov

Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University

Email: zefirovtl@mail.ru
ORCID iD: 0000-0001-9557-1639
SPIN-code: 5267-1350

Dr. Sci. (Biol.), Prof., Head of Depart., Depart. of Human Health Protection

Russian Federation, Kazan

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2. Fig. 1. The effect of neuropeptide Y (NPY; 10–7 М) on the parameters of isometric contraction of myocardial strips with preserved sinus node and spontaneous contraction in 7-day-old animals; a — original recording; b — effect in percentage; *reliability of changes compared to initial values

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3. Fig. 2. The effect of neuropeptide Y (NPY; 10–6 M) on the parameters of isometric contraction of myocardial strips with preserved sinus node and spontaneous contraction in 7-day-old animals; a — original recording; b — effect in percentage; *reliability of changes compared to initial values

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4. Рис. 3. Влияние нейропептида Y (NPY; 10–7 М) на параметры изометрического сокращения полосок миокарда с сохранённым синусовым узлом и спонтанным сокращением у 21-дневных животных; а — оригинальная запись; b — эффект в процентах; *достоверность изменений по сравнению с исходными значениями

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5. Fig. 4. The effect of neuropeptide Y (NPY; 10–6 M) on the parameters of isometric contraction of myocardial strips with preserved sinus node and spontaneous contraction in 21-day-old animals; a — original recording; b — effect in percentage; *reliability of changes compared to initial values

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6. Fig. 5. The effect of neuropeptide Y (NPY; 10–7 M) on the parameters of isometric contraction of myocardial strips with preserved sinus node and spontaneous contraction in 100-day-old rats; a — original recording; b — effect in percentage; *reliability of changes compared to initial values

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