Salivary growth factors in patients with chronic periodontitis

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Abstract

Aim. To determine the clinical value of the growth factors concentration in the oral fluid in patients with mild chronic periodontitis.

Methods. A prospective study including 30 patients with chronic periodontitis and 20 healthy volunteers was conducted. The diagnosis was made based on standard clinical and radiological criteria. Nerve growth factor β (NGF-β), hepatocyte growth factor (HGF), epidermal growth factor (EGF), vascular endothelial growth factor A (VEGF-A), platelet-derived growth factor BB (PDGF-BB) were determined in oral fluid samples by using multiparametric fluorescence analysis with magnetic microspheres (xMAP technology, Luminex 200, USA). Statistical analysis was performed using nonparametric measures: median (Me) and interquartile range (Q1, Q3). Receiver operating characteristic (ROC) analysis was used to determine the clinical value of the parameters.

Results. The chronic periodontitis was accompanied by an increase in the level of nerve growth factor-β by 2.2 times, epidermal growth factor by 3 times, vascular endothelial growth factor A by 1.9 times (p <0.05) compared with the control. The platelet-derived growth factor BB concentration did not change. Using the ROC analysis, diagnostic sensitivity and diagnostic specificity of the studied parameters were determined: 89.1 and 91.1% for nerve growth factor β, 92.3 and 96.1% for epidermal growth factor, 87.1 and 95.3% for vascular endothelial growth factor A, respectively.

Conclusion. Salivary growth factors (nerve growth factor β, epidermal growth factor, vascular endothelial growth factor A) can be considered as potential biomarkers of mild chronic periodontitis.

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About the authors

V V Bazarnyi

Ural State Medical University

Email: vlad-bazarny@yandex.ru
Russian Federation, Yekaterinburg, Russia

L G Polushina

Ural State Medical University

Author for correspondence.
Email: polushina-larisa@bk.ru
Russian Federation, Yekaterinburg, Russia

E A Sementsova

Ural State Medical University

Email: vanevs@mail.ru
Russian Federation, Yekaterinburg, Russia

A Yu Maksimova

Ural State Medical University

Email: oreshek92@list.ru
Russian Federation, Yekaterinburg, Russia

E N Svetlakova

Ural State Medical University

Email: svet_anel11@mail.ru
Russian Federation, Yekaterinburg, Russia

Yu V Mandra

Ural State Medical University

Email: jmandra@mail.ru
Russian Federation, Yekaterinburg, Russia

References

  1. Gross A.J., Paskett K.T., Cheever V.J., Lipsky M.S. Periodontitis: a global disease and the primary care provi­der's role. Postgrad Med. J. 2017; 93 (1103): 560–565. doi: 10.1136/postgradmedj-2017-134801.
  2. Bazarnyy V.V., Polushina L.G., Maksimova A.Yu., Svetlakova E.N., Mandra Yu.V. Pathogenetic substantiation of new approaches to assessing the state of oral cavity tissues in chronic generalized periodontitis. Problemy stomatologii. 2018; 14 (4): 14–18. (In Russ.) doi: 10.18481/2077-7566-2018-14-4-14-18.
  3. Polushina L.G., Svetlakova E.N., Sementsova E.A., Mandra Yu.V., Bazarnyi V.V. Clinico-pathogenetic value of some cytokines in periodontitis. Medical Immunology. 2017; 19 (6): 803–806. (In Russ.) doi: 10.15789/1563-0625-2017-6-803-806.
  4. Almehmadi A.H., Alghamdi F. Biomarkers of alveolar bone resorption in gingival crevicular fluid: A systema­tic review. Arch. Oral Biol. 2018; 93: 12–21. doi: 10.1016/j.archoralbio.2018.05.004.
  5. Lorenzo-Pouso A.I., Pérez-Sayáns M., Bravo S.B., López-Jornet P., García-Vence M., Alonso-Sampedro M., Carballo J., García-García A. Protein-based salivary profiles as novel biomarkers for oral diseases. Dis. Markers. 2018; 2018: 6141845. doi: 10.1155/2018/6141845.
  6. Bazarny V.V., Polushina L.G., Sementsova E.A., Svetlakova E.N., Beresneva N.S., Mandra Yu.V., Tsvirenko S.V. The interleukins in the pathoge­nesis of periodontitis. Vestnik Ural''skoy meditsinskoy akademicheskoy nauki. 2017; 14 (1): 35–39. (In Russ.)
  7. Li S., Gu X., Yi S. The regulatory effects of transforming growth factor-β on nerve regeneration. Cell Transplant. 2017; 26 (3): 381–394. doi: 10.3727/096368916X693824.
  8. Afacan B., Öztürk V.Ö., Paşalı Ç., Bozkurt E., Köse T., Emingil G. Gingival crevicular fluid and salivary HIF-1α, VEGF, and TNF-α levels in periodontal health and disease. J. Periodontol. 2019; 90 (7): 788–797. doi: 10.1002/JPER.18-0412.
  9. Angiero F., Ugolini A., Cattoni F., Bova F., Blasi S., Gallo F., Cossellu G., Gherlone E. Evaluation of bradykinin, VEGF, and EGF biomarkers in gingival crevicular fluid and comparison of PhotoBioModulation with conventional techniques in periodontitis: a split-mouth randomized clinical trial. Lasers Med. Sci. 2020; 35 (4): 965–970. doi: 10.1007/s10103-019-02919-w.
  10. Choi Y., Yoo J.H., Lee J.H., Lee Y., Bae M.K., Kim Y.D., Kim H.J. Connective tissue growth factor (CTGF) regulates the fusion of osteoclast precursors by inhibiting Bcl6 in periodontitis. Int. J. Med. Sci. 2020; 17 (5): 647–656. doi: 10.7150/ijms.41075.
  11. Smith P.C., Martínez C., Cáceres M., Martínez J. Research on growth factors in periodontology. Periodontol. 2000. 2015; 67 (1): 234–250. doi: 10.1111/prd.12068.
  12. Zekeridou A., Mombelli A., Cancela J., Courvoisier D., Giannopoulou C. Systemic inflammatory burden and local inflammation in periodontitis: What is the link between inflammatory biomarkers in serum and gingival crevicular fluid? Clin. Exp. Dent. Res. 2019; 5 (2): 128–135. doi: 10.1002/cre2.162.
  13. Guru S., Sam S.E., Rajan S., Padmanabhan S. Comparative evaluation of salivary hepatocyte growth factor in smokers and non-smokers with chronic periodontitis. J. Investig. Clin. Dent. 2018; 9 (2): e12306. doi: 10.1111/jicd.12306.
  14. Romano F., Bongiovanni L., Bianco L., Di Scipio F., Yang Z., Sprio A.E., Berta G.N., Aimetti M. Biomarker ­levels in gingival crevicular fluid of generalized aggressive periodontitis patients after non-surgical periodontal treatment. Clin. Oral Investig. 2018; 22 (2): 1083–1092. doi: 10.1007/s00784-017-2192-1.
  15. Li W., Zhang Z., Wang Z. Differential immune cell infiltrations between healthy periodontal and chronic periodontitis tissues. BMC Oral Health. 2020; 20 (1): 293. doi: 10.1186/s12903-020-01287-0.
  16. Taskan M.M., Karatas O., Balci Yuce H., Isiker Kara G., Gevrek F., Ucan Yarkac F. Hypoxia and collagen crosslinking in the healthy and affected sites of periodontitis patients. Acta Odontol. Scand. 2019; 77 (8): 600–607. doi: 10.1080/00016357.2019.1624819.
  17. Coelho J.M.F., Miranda S.S., da Cruz S.S., Trindade S.C., Passos-Soares J.S., Cerqueira E.M.M., Costa M.D.C.N., Figueiredo A.C.M.G., Hintz A.M., Barreto M.L., Seymour G.J., Scannapieco F., Gomes-Filho I.S. Is there association between stress and periodontitis? Clin. Oral Investig. 2020; 24 (7): 2285–2294. doi: 10.1007/s00784-019-03083-9.
  18. Haririan H., Andrukhov O., Böttcher M., Pablik E., Wimmer G., Moritz A., Rausch-Fan X. Salivary neuropeptides, stress, and periodontitis. J. Periodontol. 2018; 89 (1): 9–18. doi: 10.1902/jop.2017.170249.
  19. Jasim H., Ghafouri B., Carlsson A., Hedenberg-Magnusson B., Ernberg M. Daytime changes of sa­livary biomarkers involved in pain. J. Oral Rehabil. 2020; 47 (7): 843–850. doi: 10.1111/joor.12977.

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