Studying the blood clotting investigation in prescence of boron-dipyrrin fluorescent dyes
- Authors: Aleksakhina EL1, Marfin Y.S2, Merkushev DA2, Tomilova IK1, Rumyantsev EV2
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Affiliations:
- Ivanovo State Medical Academy, Ivanovo, Russia
- Ivanovo State University of Chemistry and Technology, Ivanovo, Russia
- Issue: Vol 96, No 5 (2015)
- Pages: 792-798
- Section: Experimental medicine
- URL: https://kazanmedjournal.ru/kazanmedj/article/view/2029
- DOI: https://doi.org/10.17750/KMJ2015-792
- ID: 2029
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Full Text
Abstract
Aim. To study the blood clotting in prescence of boron-dipyrrin fluorescent dyes.
Methods. Photophysical properties of several boron-dippiryn based fluorescent dyes were examined in presence of blood plasma biomolecules and in model system containing bovine serum albumin using electron absorption spectroscopy and fluorescence spectroscopy.
Results. The interaction between the investigated dyes and protein plasma components changes spectral characteristics of the dyes and leads to bathochromic and hypochromic shifts of absorption spectra accompanied by changing of fluorescence intensity. The mechanism of fluorescence changing was defined within the Stern-Folmer theory. It was shown that the static factor prevails due to dye-biopolymers molecular complex formation at plasma protein concentration up to 1 g/l, while the higher levels are characterized mainly by nonspecific interactions of fluorophores. The increase of fluorescent characteristics of phenyl-substituted BODIPY in the presence of proteins caused by resonance energy transfer and physicochemical features changes of the fluorophore molecular environment was shown.
Conclusion. The gained results demonstrate the possibility of using the BODIPY dye for determination of blood clotting activity. Specific interactions of dye molecules with studied biological objects allows to analyze the contents and dynamic processes in biological objects at blood serum clotting.
Keywords
About the authors
E L Aleksakhina
Ivanovo State Medical Academy, Ivanovo, Russia
Author for correspondence.
Email: ymarfin@gmail.com
Yu S Marfin
Ivanovo State University of Chemistry and Technology, Ivanovo, Russia
Email: ymarfin@gmail.com
D A Merkushev
Ivanovo State University of Chemistry and Technology, Ivanovo, Russia
Email: ymarfin@gmail.com
I K Tomilova
Ivanovo State Medical Academy, Ivanovo, Russia
Email: ymarfin@gmail.com
E V Rumyantsev
Ivanovo State University of Chemistry and Technology, Ivanovo, Russia
Email: ymarfin@gmail.com
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