The role of membrane lipids in forming the electrokinetic properties of red blood cells in solid tumors and psoriasis

Cover Page

Cite item


Aim. Analysis of the electrophoretic mobility of red blood cells in patients with the diseases caused by increased cell proliferation activity, and its relationship with the lipid spectrum of erythrocyte membranes.

Methods. The blood of 42 patients with malignant tumors of epithelial tissues (colon, kidney, prostate, urine bladder and cervical cancer) and 16 patients with skin diseases was studied. The control group consisted of 20 healthy individuals. Electrophoretic mobility of red blood cells was assessed by microelectrophoresis. Lipid fractions levels in erythrocytes was determined by one-dimensional thin layer chromatography.

Results. Over than two-fold decrease of electrophoretic mobility of erythrocytes was observed in patients with malignant tumors of epithelial tissues and skin diseases associated with cell proliferation processes (psoriasis) - by 59 and 60%, respectively In inflammatory skin diseases (microbial eczema), the decrease was less pronounced (30%). The contents of charged phospholipid in erythrocyte membranes in case of cancer and psoriasis has changed in a similar way: a significant reduction in sphingomyelin level in the absence of significant differences in phosphatidylethanolamine and phosphatidylcholine levels. Multidirectional statistically significant changes in the contents of lysophosphatidylcholine were revealed depending on the stage of cancer, their level was decreased two-fold in case of psoriasis. The changes of cholesterol, diacylglycerols, free fatty acids levels in the membranes of red blood cells in case of cancer and psoriasis were divergent.

Conclusion. In diseases caused by cell proliferation activation, electrophoretic mobility of erythrocytes is reduced by more than 50%. Kinetic characteristic of erythrocytes may be used for detecting the conditions of pathologic cell proliferation, including malignant tumors, since they alter in cases of phospholipid membranes lipid spectrum disorders.

About the authors

L M Obukhova

Nizhny Novgorod State Medical Academy, Nizhny Novgorod, Russia

Author for correspondence.

E I Erlykina

Nizhny Novgorod State Medical Academy, Nizhny Novgorod, Russia


T V Kopytova

Nizhny Novgorod State Medical Academy, Nizhny Novgorod, Russia


M N Makarova

Nizhny Novgorod State Medical Academy, Nizhny Novgorod, Russia


N A Shchelchkova

Nizhny Novgorod State Medical Academy, Nizhny Novgorod, Russia


E S Beschastnova

Volga District Medical Centre under Federal Medical and Biological Agency, Nizhny Novgorod, Russia



  1. Бакулев А.Л., Шагова Ю.В., Козлова И.В. Псориаз как системная патология // Саратов. науч.-мед. ж. - 2008. - Т. 4, №1. - С. 13-20.
  2. Боровская М.К., Кузнецова Э.Э., Горохова В.Г. и др. Структурно-функциональная характеристика мембраны эритроцита и её изменения при патологиях разного генеза // Бюлл. ВС НЦ СО РАМН. - 2010. - Т. 73, №3. - С. 334-354.
  3. Бутов Ю.С., Хрусталёва Е.А., Фёдорова Е.Г. Влияние липидстабилизирующей терапии на показатели иммунной реактивности и липидного обмена у больных псориазом // Рос. ж. кожн. и вен. болезней. - 1999. - №6. - С. 24-26.
  4. Крылов В.Н., Дерюгина А.В. Типовые изменения электрофоретической подвижности эритроцитов при стрессовых воздействиях // Бюлл. эксп. биол. и мед. - 2005. - Т. 139, №4. - С. 364-366.
  5. Новицкий В.В., Рязанцева Н.В., Степовая Е.А. и др. Молекулярные нарушения мембраны эритроцитов при патологии разного генеза являются типовой реакцией организма: контуры проблемы // Бюлл. сибир. мед. - 2006. - Т. 5, №2. - С. 62-69.
  6. Титов В.Н., Лисицын Д.М. Жирные кислоты. Физическая химия, биология и медицина. - М., Тверь: Триада, 2006. - 672 с.
  7. Харамоненко С.С., Ракитянская А.А. Электрофорез клеток крови в норме и при патологии. - Минск: Беларусь, 1974. - 144 с.
  8. Bessho F. Effects of mass screening on age specific incidence of neuroblastoma // Int. J. Cancer. - 1996. - Vol. 67, N 4. - P. 520-522.<520::AID-IJC10>3.0.CO;2-B
  9. Claria J., Arroyo V. Prostaglandins and other cyclooxygenase-dependent arachidonic acid metabolites and the kidney in liver disease // Prostaglandins Other Lipid Mediat. - 2003. - Vol. 72, N 1-2. - P. 19-33.
  10. Merrill A.H.Jr, Jones D.D. An update of the enzymology and regulation of sphingomyelin metabolism // Biochim. Biophys. Acta. - 1990. - Vol. 1044. - P. 1-12.
  11. Petelska A.D., Figaszewski Z.A. Effect of pH on the interfacialtension of bilayer lipid membrane formed from phosphotidylcholine or phosphatidylserine // Biochem., Biophys. Acta. - 2002. - Vol. 1561. - Р. 131-146.
  12. Torkhovskaya T.V, Ipatova O.M., Medvedeva N.V. et al. Membrane proteins and phospholipids as effectors of reverse cholesterol transport // Biochemistry (Moscow). Supplement Series B: Biomed. Chem. - 2006. - Vol. 52, N 2. - P. 113-123.

© 2015 Obukhova L.M., Erlykina E.I., Kopytova T.V., Makarova M.N., Shchelchkova N.A., Beschastnova E.S.

Creative Commons License

This work is licensed
under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies