Microanatomy of vitreoretinal interactions in macular and paramacular areas

Abstract


Aim. To refine microanatomy of vitreoretinal interactions in central areas of retina and to obtain additional information on vitreoretinal interactions regarding theoretical possibility of collagen synthesis in the vitreous body of adult humans and mechanisms of vitreoretinal adhesion.

Methods. Scanning electron microscopy study was performed in central areas of human retina after removal of vitreous base from its surface.

Results. On the surface of retina we found a thin layer of epiretinal vitreous body most strongly bound to the retina. Thickness of paramacular epiretinal vitreous is 3–5 µm and it is formed mainly by thin densely packed fibrils. Its surface layer facing the vitreous cavity consists of the thickest (1 µm) and longest (250 µm) fibrils loosely packed. These fibrils look like “pearl threads” and exhibit signs of newly formed fibrils. Epiretinal layer of the vitreous in the fovea is thinner, its thickness is about 2 µm. Top layer of fibrils is least densely packed with the thickness of the fibrils of about 0.2 µm. The closer to internal limiting membrane, the thinner the fibrils, the denser their packing. The discovered layer can be interpreted as an additional anatomic structural unit of the vitreous body — epiretinal vitreous.

Conclusion. We detected indirect signs of lifetime synthesis of newly formed fibrils in the vitreous of adult human; newly formed collagen may be localized in epiretinal vitreous which may be the main binding component providing vitreoretinal adhesion.


P V Lyskin

S.N. Fyodorov Eye Microsurgery Federal State Institution of the Russian Ministry of Health

Author for correspondence.
Email: plyskin@yahoo.com
Moscow, Russia

  1. Foos R.Y. Vitreoretinal juncture; topographical variations. Invest. Ophthalmol. Vis. Sci. 1972; 11: 801–808. PMID: 4561129.
  2. Foos R.Y., Wheeler N.C. Vitreoretinal juncture. Synchysis senilis and posterior vitreous detachment. Ophthalmology. 1982; 89: 1502–1512. doi: 10.1016/S0161-6420(82)34610-2.
  3. Heegaard S. Structure of the human vitreoretinal border region. Ophthalmologica. 1994; 208: 82–91. doi: 10.1159/000310458.
  4. Sebag J. Anatomy and pathology of the vitreo-retinal interface. Eye. 1992; 6: 541–542. doi: 10.1038/eye.1992.119.
  5. Sebag J. The vitreous: structure, function, and patho­bio­logy. Springer. 1989; 173. doi: 10.1007/978-1-4613-8908-8.
  6. Sebag J., Balazs E.A. Morphology and ultrastructure of human vitreous fibers. Invest. Ophthalmol. Vis. Sci. 1989; 30: 1867–1871.
  7. Snead M.P., Snead D.R., Mahmood A.S., Scott J.D. Vitreous detachment and the posterior hyaloid membrane: a Clinicopathological study. Eye. 1994; 8: 204–209. doi: 10.1038/eye.1994.47.
  8. Snead M.P., Snead D.R., Richards A.J. Clinical, histological and ultrastructural studies of the posterior hyaloid membrane. Eye. 2002; 16: 447–453. doi: 10.1038/sj.eye.6700198.
  9. Balazs E. Molecular morphology of the vitreous body. The structure of the eye. New York: Academic Press. 1961; 295.
  10. Sang D.N. Embryology of the vitreous. Congenital and developmental abnormalities. Bull. Soc. Belge. Ophtalmol. 1987; 223 (1): 11–35. doi: 10.1007/978-1-4757-1901-7_2.
  11. Sebag J. Vitreous in health and disease. Springer. 2014: 4–7. doi: 10.1007/978-1-4939-1086-1.
  12. Balazs E.A. Structure of vitreous gel. In: Acta XVII concilium ophthalmologicum. 1954; 11: 1019.
  13. Balazs E.A. Studies on structure of vitreous body: absorption of ultraviolet light. Am. J. Ophthalmol. 1954; 38: 21. doi: 10.1016/0002-9394(54)90005-7.
  14. Balazs E.A. Functional anatomy of the vitreus. In: Ocular anatomy, embryology and teratology. F. Jacobiec, editor. New York: Harper and Row. 1982; 425–440.
  15. Balazs E.A., Denlinger J.L. Aging and human visual function. New York: Alan R. Liss. 1982; 45–57.
  16. Cho E.H., Ku H.C., Il W., Lee E.K. Residual vitreous cortex at the fovea during vitrectomy for primary rhegmatogenous RD repair. Retina. 2018; 38 (8):1549–1555. doi: 10.1097/IAE.0000000000001734.

Views

Abstract - 20

PDF (Russian) - 18

Cited-By


PlumX


© 2019 Lyskin P.V.

Creative Commons License

This work is licensed
under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.


Свидетельство о регистрации СМИ ЭЛ № ФС 77-75008 от 1 февраля 2019 года выдано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор)