Inhibitory effect of DL-butionine sulfoximine on P-glycoprotein activity in vitro

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Abstract

Background. P-glycoprotein (Pgp) is a multidrug resistance protein 1 with broad substrate specificity. Its functioning can change under the influence of various substances, so the search for endogenous and exogenous compounds that modulate the activity of the transporter protein is an important area of research.

Aim. To evaluate the effect of DL-butionine sulfoximine on the activity and amount of the Pgp transporter protein in Caco-2 cells.

Material and methods. The study was performed on a human colon adenocarcinoma cell line (Caco-2). Cells were incubated with DL-butionine sulfoximine and quinidine (a classical Pgp inhibitor) at concentrations of 1, 5, 10, 50, 100, and 500 µM for 3 hours. Pgp activity was evaluated by the transport of its substrate, fexofenadine, which concentration was determined by high performance liquid chromatography with ultraviolet detection (Stayer, Russia). The results were analyzed using StatSoft Statistica 13.0 (ANOVA), IC50 was calculated using GraphPad Prism 8 software. Differences were considered statistically significant at p <0.05.

Results. Incubation with DL-butionine sulfoximine and quinidine at concentrations of 1–500 µM for 3 hours did not affect the amount of Pgp in Caco-2 cells. Pgp activity decreased when using DL-butionine sulfoximine at concentrations of 50–500 µM by a maximum of 47.7% (p=0.040). Quinidine at concentrations of 5–500 µM reduced Pgp activity by a maximum of 79.1% (p=0.0002) at a concentration of 500 µM. Quinidine inhibited Pgp activity at lower concentrations compared to DL-butionine sulfoximine: the IC50 of fexofenadine with quinidine was 5.16±0.59 µmol/l, for DL-butionine sulfoximine it was 17.21±2.46 µmol/l (p= 0.001).

Conclusion. DL-butionine sulfoximine has a direct inhibitory effect on the activity of the Pgp transporter protein on the Caco-2 cell line.

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About the authors

Yulia V. Abalenikhina

Ryazan State Medical University named after I.P. Pavlova

Author for correspondence.
Email: abalenihina88@mail.ru
ORCID iD: 0000-0003-0427-0967

Cand. Sci. (Biol.), Assoc. Prof., Depart. of Biological Chemistry

Russian Federation, Ryazan, Russia

Pavel Yu. Mylnikov

Ryazan State Medical University named after I.P. Pavlova

Email: pavelmylnikov@mail.ru
ORCID iD: 0000-0001-7829-2494
SPIN-code: 8503-3082

PhD-Student, Depart. of Pharmacology with a Course of Pharmacy

Russian Federation, Ryazan, Russia

Alexey V. Shchulkin

Ryazan State Medical University named after I.P. Pavlova

Email: alekseyshulkin@rambler.ru
ORCID iD: 0000-0003-1688-0017
SPIN-code: 2754-1702

M.D., D. Sci. (Med.), Prof., Depart. of Pharmacology with a Course of Pharmacy, Continuing Professional Education Faculty

Russian Federation, Ryazan, Russia

Elena N. Yakusheva

Ryazan State Medical University named after I.P. Pavlova

Email: enya.rzn@yandex.ru
ORCID iD: 0000-0001-6887-4888
SPIN-code: 2865-3080

M.D., D. Sci. (Med.), Prof., Head of Depart. of Pharmacology with a Course of Pharmacy, Continuing Professional Education Faculty

Russian Federation, Ryazan, Russia

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1. JATS XML
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2. Рис. 1. Структура трансвелл-системы

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3. Рис. 2. Относительное количество Р-гликопротеина (Pgp) в клетках линии Сасо-2 при воздействии DL-бутионинсульфоксимина (БСО) и хинидина в концентрациях 1–500 мкМ в течение 3 ч; К — контроль; GAPDH — глицеральдегид-3-фосфатдегидрогеназа

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4. Рис. 3. Изменение коэффициента кажущейся проницаемости b-a (Papp b-a, а) и отношения коэффициентов кажущейся проницаемости (Рарр b-a/Рарр a-b, б) фексофенадина под действием DL-бутионинсульфоксимина (БСО) и хинидина в концентрациях 1–500 мкМ; IC50 — концентрация полумаксимального ингибирования. Расчёт IC50 и построение графиков выполнены с использованием программы GraphPad Prism 8

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