Proliferative-apoptotic features of rat colon epithelium after electron irradiation

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Abstract

BACKGROUND: One of the promising types of radiation therapy for colon malignant neoplasms is electron irradiation.

AIM: Morphological assessment of apoptosis and proliferation of animals’ colon epithelium after local fractional irradiation with electrons in a total focal dose of 25 Gy.

MATERIAL AND METHODS: Wistar rats were used, which were divided into two groups: the first — control (n=10); the second — experimental (n=20). Animals of the experimental group were subjected to local fractional irradiation with electrons in a total focal dose of 25 Gy (in fractions of 5 Gy on the 1st, 3rd, 5th, 7th, 9th day of irradiation). Fragments of the colon were examined by light microscopy, immunohistochemical reactions were carried out with antibodies to Ki-67 and Cas3. The wall thickness and the diameter of the serous-mucous membrane were measured and calculated in micrometers. Morphological changes were evaluated in points. The results were assessed using the Kolmogorov–Smirnov test, Student's t-test, Mann–Whitney U-test and Fisher's test.

RESULTS: Macroscopic examination of the colon of rats in the experimental group revealed hyperemia of the mucous membrane without significant destructive changes. Microscopic examination revealed minor damage to the mucous membrane of the colon compared to the control group, a reduction in goblet cells (63.17±1.87% versus 21.64±1.37%, p=0.009) was noted. The number of caspase-3-positive epithelial cells increased (28.7±8.2% versus 14.5±3.9%, p=0.027). The proliferative index (Ki-67) in the intestinal crypts of the colon after local electron irradiation with a total focal dose of 25 Gy was reduced compared to the control group (11.14±6.23% versus 19.45±5.12%, p=0.013).

CONCLUSION: With local fractional electron irradiation of rats in a total focal dose of 25 Gy, weak degenerative and dystrophic changes in the epithelium, reduction of goblet cells with preservation of regenerative potential occur in the colon.

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About the authors

Susanna V. Saakian

Crimean Federal University named after V.I. Vernadsky

Email: drsaakyan@icloud.com
ORCID iD: 0000-0001-8606-8716
SPIN-code: 7742-1420

Postgrad. Stud., Depart. of Histology, Medical Academy named after S.I. Georgievsky

Russian Federation, Simferopol

Alexandr F. Mimuni

First Moscow State Medical University named after I.M. Sechenov (Sechenov University)

Email: a.mimuni@yandex.ru
ORCID iD: 0009-0004-7547-3790

student

Russian Federation, Moscow

Elza B.-G. Karakayeva

Crimean Federal University named after V.I. Vernadsky

Email: kchr09@mail.ru
ORCID iD: 0000-0001-9833-3433
SPIN-code: 8221-3003

Postgrad. Stud., Depart. of Histology, Medical Academy named after S.I. Georgievsky

Russian Federation, Simferopol

Konstantin K. Gotovtsev

First Moscow State Medical University named after I.M. Sechenov (Sechenov University)

Email: kostya.gotovtsev@gmail.com
ORCID iD: 0009-0008-6862-5900

student

Russian Federation, Moscow

Grigory A Demyashkin

First Moscow State Medical University named after I.M. Sechenov (Sechenov University)

Author for correspondence.
Email: dr.dga@mail.ru
ORCID iD: 0000-0001-8447-2600
SPIN-code: 5157-0177

MD, Dr. Sci. (Med.), Head of Laboratory, Laboratory of Histology and Immunohistochemistry

Russian Federation, Moscow

References

  1. Bentzen SM. Preventing or reducing late side effects of radiation therapy: Radiobiology meets molecular pathology. Nat Rev Cancer. 2006;6(9):702–713. doi: 10.1038/nrc1950
  2. Birgisson H, Påhlman L, Gunnarsson U, Glimelius B. Late adverse effects of radiation therapy for rectal cancer — a systematic overview. Acta Oncol. 2007;46(4):504–516. doi: 10.1080/02841860701348670
  3. Bertholet J, Knopf A, Eiben B, McClelland J, Grimwood A, Harris E, Menten M, Poulsen P, Trang Nguyen D, Keall P, Oelfke U. Real-time intrafraction motion monitoring in external beam radiotherapy. Phys Med Biol. 2019;64(15):15TR01. doi: 10.1088/1361-6560/ab2ba8
  4. Fiorino C, Valdagni R, Rancati T, Sanguineti G. Dose-volume effects for normal tissues in external radiotherapy: Pelvis. Radiother Oncol. 2009;93(2):153–167. doi: 10.1016/j.radonc.2009.08.004
  5. Kim J, Jenrow K, Brown S. Mechanisms of radiation-induced normal tissue toxicity and implications for future clinical trials. Radiat Oncol J. 2014;32(3):103–115. doi: 10.3857/roj.2014.32.3.103
  6. Zharikov GM, Vinokurov VL, Zaikin GV. Radiation injuries of the rectum and bladder in patients with cervical cancer. Mir meditsiny. 2000;2(7–8):17–21. (In Russ.)
  7. Andreyev H. Gastrointestinal problems after pelvic radiotherapy: The past, the present and the future. Clin Oncol. 2007;19(10):790–799. doi: 10.1016/j.clon.2007.08.011
  8. Lu L, Li W, Chen L, Su Q, Wang Y, Guo Z, Lu Y, Liu B, Qin S. Radiation-induced intestinal damage: Latest molecular and clinical developments. Future Oncol. 2019;15(35):4105–4118. doi: 10.2217/fon-2019-0416
  9. McBride W, Schaue D. Radiation-induced tissue damage and response. J Pathol. 2020;250(5):647–655. doi: 10.1002/path.5389
  10. Ahmed M, Ahmed R. Radiation in gastroenterology. Gastroenterol Res. 2022;15(6):285–296. doi: 10.14740/gr1567
  11. Kam W, Banatia R. Effects of ionizing radiation on mitochondria. Free Radic Biol Med. 2013;65:607–619. doi: 10.1016/j.freeradbiomed.2013.07.024
  12. Fedyanin M, Artamonova E, Barsukov Yu, Bolotina L, Gladkov O, Glebovskaya V, Gordeev S, Karachun A, Kozlov N, Lyubchenko L, Malikhova O, Mamedli Z, Mikhailov A, Podluzhny D, Protsenko S, Rybakova I, Samsonov D, Sidorov D, Snegovoy A, Tkachev S, Tryakin A, Tsukanov A, Chernykh M, Shelygin Yu. Practical recommendations on the medicinal effects of rectal cancer. Practical recommendations. Zlokachestvennye opukholi. 2020;10(3s2-1):391–438. (In Russ.) doi: 10.18027/2224-5057-2020-10-3s2-23
  13. Kasprzak A. Prognostic biomarkers of cell proliferation in colorectal cancer (CRC): From immunohistochemistry to molecular biology techniques. Cancers. 2023;15(18):4570. doi: 10.3390/cancers15184570
  14. Olsson M, Zhivotovsky B. Caspases and cancer. Cell Death Differ. 2011;18(9):1441–1449. doi: 10.1038/cdd.2011.30
  15. Kim C, Yang V, Bialkowska A. The role of intestinal stem cells in epithelial regeneration following radiation-induced gut injury. Curr Stem Cell Rep. 2017;3(4):320–332. doi: 10.1007/s40778-017-0103-7
  16. Figueiredo J, Passarelli M, Wei W, Ahnen D, Morris J, Corley L, Mehta T, Bartley A, McKeown-Eyssen G, Bresalier R, Barry E, Goel A, Mesa G, Hamilton S, Baron J. Proliferation, apoptosis and their regulatory protein expression in colorectal adenomas and serrated lesions. PLoS ONE. 2021;16(11):e0258878. doi: 10.1371/journal.pone.0258878
  17. Andrés-Sánchez N, Fisher D, Krasinska L. Physiological functions and roles in cancer of the proliferation marker Ki-67. J Cell Sci. 2022;135(11):jcs258932. doi: 10.1242/jcs.258932
  18. Gu Q, Jiao S, Duan K, Wang Y, Petralia R, Li Z. The BAD-BAX-caspase-3 cascade modulates synaptic vesicle pools via autophagy. J Neurosci Off J Soc Neurosci. 2021;41(6):1174–1190. doi: 10.1523/JNEUROSCI.0969-20.2020
  19. Kanth P, Rajan T. Chromogranin A and Ki67 marker in normal colon, serrated polyp and colorectal tubular adenoma. Off J Am Coll Gastroenterol ACG. 2011;106:S562. doi: 10.14309/00000434-201110002-01470

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