Endogenous sodium pump inhibitors: common pathogenetic mechanisms of impaired glucose tolerance and preeclampsia

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Aim. Study of the influence of sodium chloride overconsumption by pregnant rats with induced diabetes mellitus on the synthesis of marinobufagenin and Na/K-ATPase activity. Methods. The study was performed on 72 female Sprague Dawley rats. Type 2 diabetes mellitus was induced by administration of 65 mg/kg streptozotocin on day 4 after birth. Increased intake of salt was achieved by replacement of drinking water with 1.8% sodium chloride solution on days 12 to 19 of pregnancy. Results. In intact rats, pregnancy was associated with a two-fold increase of marinobufagenin level in the blood and mild impairment of glucose tolerance. Pregnant rats with induced diabetes mellitus apart from fetal macrosomia exhibited greater impairment of glucose tolerance and higher levels of marinobufagenin compared to those in intact pregnant animals. Compared to healthy pregnant rats, salt supplementation for laboratory animals with diabetes was associated with an increase in systolic blood pressure, decreased fetal and placental weight, five-fold elevation of marinobufagenin excretion, and in 42% - with inhibition of Na/K-ATPase activity in erythrocytes. In nonpregnant rats, pre-treatment with anti-marinobufagenin antibodies produced an exaggerated increase of blood levels of glucose and insulin in oral glucose tolerance test. Conclusion. Marinobufagenin is an important factor of pathogenesis of both preeclampsia and diabetes mellitus, and regulation of glucose tolerance may be one of the physiological functions of endogenous cardiotonic steroids.

About the authors

V A Reznik

Saint Petersburg State Pediatric Medical Academy

Email: tapnatalia@yandex.ru
Saint Petersburg, Russia

O V Fedorova

National Institute of Aging, National Institute of Health

Email: tapnatalia@yandex.ru
Baltimore, USA

N I Tapil’skaya

Saint Petersburg State Pediatric Medical Academy

Email: tapnatalia@yandex.ru
Saint Petersburg, Russia

A Ya Bagrov

National Institute of Aging, National Institute of Health; I.M. Sechenov Institute of Evolutionary Physiology and Biochemistry

Email: tapnatalia@yandex.ru
Baltimore, USA; Saint Petersburg, Russia


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© 2017 Reznik V.A., Fedorova O.V., Tapil’skaya N.I., Bagrov A.Y.

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