Features of coronary artery disease in patients with different genotypes of PAI-1 gene

Aim. To investigate the relation of different genotypes and alleles of PAI-1 gene with the course of coronary artery disease.

Methods. We observed 80 patients with coronary artery disease (51 males and 29 females) aged 39 to 65 years living in the city of Baku. Genotyping of a polymorphic marker 4G(-675)5G was performed by means of polymerase chain reaction followed by restriction fragment length analysis. Genomic deoxyribonucleic acid (DNA) was extracted from venous blood leukocytes. To determine the frequency distribution of genotypes and alleles of PAI-1 in ischemic heart disease all patients were divided into 6 groups depending on the course of the disease and its outcomes.

Results. In the population of Baku genotype 44 of PAI-1 gene is significantly more common in people with coronary artery disease compared to individuals without coronary artery disease and is a risk factor for this disease. Genotype 55 of PAI-1 gene is more common in people without coronary artery disease and thus is a protective genotype against this disease. Allele 4 of PAI-1 was statistically more frequently recorded in patients with ischemic heart disease, and allele 5 was significantly more frequently observed in patients without this pathology. Therefore, allele 4 is a risk factor for the occurrence of coronary heart disease and allele 5 is protective against this disease. Alleles 5 and 4 are not associated with the course and outcomes of ischemic heart disease.

Conclusion. According to the results of the study on the population of Baku, genotype 44 of PAI-1 gene is a risk factor of ischemic heart disease, genotype 55 of PAI-1 gene is protective against this pathology, allele 4 is characteristic for people with coronary artery disease (a risk factor), allele 5 is characteristic for people without it (protective role) whereas the named genotypes of PAI-1 are not connected to the course and outcomes of this disease.