Contractions dynamic of “fast” and “slow” rat muscle under spinal shock and modulators of contraction

Cover Page
Open Access Open Access
Restricted Access Access granted
Restricted Access Subscription or Fee Access

Abstract


Aim. To study the dynamics of neuromotor regulation of the contractile function of “fast” and “slow” muscles in rodents during spinal shock by spinal cord transection at the level Тh11–Тh12.

Methods. The experiments were carried out on laboratory rats weighing 140–180 g. The animals were divided into two groups: “Control” (8 rats) and “Spinal shock” (6 rats). The lower leg muscles, m. soleus and m. extensor digitorum longus (m. EDL), were dissected by partially isolating without disrupting the connection with the body's circulatory system. The sciatic nerve was stimulated with single electrical impulses (10 V, 0.5 ms). Contractions of both muscles caused by electrical stimulation of the sciatic nerve before and after the injection of the substances into the femoral artery — tubocurarine (1 mM) or norepinephrine (10 mM) — were recorded in animals of both groups. After spinalization, muscle contractions were re-recorded during electrical stimulation of the sciatic nerve before and 10 minutes after the injection of tubocurarine or noradrenaline into the femoral artery in the same concentrations.

Results. After spinalization of the animal, the contraction force of the muscle m. EDL fibers increased to 0.43±0.03 g (p=0.040), but the temporal parameters remained unchanged. M. soleus, on the contrary, showed a decrease in the contraction time to 0.053±0.005 s (p=0.045), and no change in the contraction force was observed under these conditions. Intra-arterial administration of norepinephrine in the control group resulted in an increase of m. soleus contractions up to 1.21±0.17 g (p=0.048), and m. EDL — up to 0.57±0.07 g (p=0.043). The administration of norepinephrine in spinalized animals caused an increase in the contraction of m. soleus up to 1.21±0.09 g (p=0.047), and m. EDL up to 0.66±0.05 g (p=0.043). The blocker of postsynaptic cholinergic receptors tubocurarine administration reduced the force of contraction of both muscle types in both control [m. soleus up to 0.39±0.03 g (p=0.039), m. EDL up to 0.11±0.02 g (p=0.042)] and spinalized [m. soleus up to 0.34±0.05 g (p=0.039), m. EDL up to 0.15±0.04 g (p=0.040)] animals.

Conclusion. The data obtained demonstrate the presence of significant differences in the mechanisms of control of contractile activity in the “fast” and “slow” skeletal muscles of warm-blooded animals; the persistence of the similar effect of the basic modulators on the contraction of both muscles with such a striking reaction to spinalization highlights the contribution of neurotrophic control to the functioning of “fast” and “slow” motor units.


Full Text

Restricted Access

About the authors

V V Valiullin

Kazan State Medical University

Email: khajrulli@ya.ru

Russian Federation, Kazan, Russia

A E Khairullin

Kazan State Medical University

Author for correspondence.
Email: khajrulli@ya.ru

Russian Federation, Kazan, Russia

A A Eremeev

Kazan Federal University

Email: khajrulli@ya.ru

Russian Federation, Kazan, Russia

A Yu Teplov

Kazan State Medical University

Email: khajrulli@ya.ru

Russian Federation, Kazan, Russia

A R Shaikhutdinova

Kazan State Medical University

Email: khajrulli@ya.ru

Russian Federation, Kazan, Russia

N M Kashtanov

Kazan State Medical University

Email: khajrulli@ya.ru

Russian Federation, Kazan, Russia

S N Grishin

Kazan State Medical University

Email: khajrulli@ya.ru

Russian Federation, Kazan, Russia

References

  1. Valiullin V.V. Neurotrophic control of skeletal muscles in hyperthyroid animals. Neurobiological issues. Nauchnye trudy KGMI. 1987; 48–53. (In Russ.)
  2. Isla­mov R.R., Valiullin V.V. Neuotrophic regulation of sceletal muscle plasticity. Nevrologicheskiy vestnik. 2014; 46 (3): 56–64. (In Russ.) doi: 10.17816/nb13874.
  3. Valiullin V.V., Islamov R.R., Valiullina M.E., Poletaev G.I. Neurotrophic control of myosin synthesis in the slow muscle of guinea pig. Bjulleten' eksperimental'noj biologii i mediciny. 1991; 111 (2): 201–203. (In Russ.)
  4. Valiullin V.V., Rezvjakov N.P. The effect of hormonal and neurotrophic factors on the expression of fast-type myosin in slow muscle. Bjulleten' eksperimental'noj biologii i mediciny. 1986; 102 (11): 521–523. (In Russ.)
  5. Khabirov F.A. The role of the disorders of neurotrophic control in vertebral neurology. Prakticheskaya meditsina. 2013; (1): 10–15. (In Russ.)
  6. Fox A.D. Spinal shock. Assessment & treatment of spinal cord injuries & neurogenic shock. JEMS. 2014; 39 (11): 64–67.
  7. Guttmann L. Spinal shock and reflex behaviour in man. Paraplegia. 1970; 8 (2): 100–116. doi: 10.1038/sc.1970.19.
  8. Hall M. Fourth memoirs on some principles of pathology in the nervous system. Med. Chir. Trans. 1841; 24: 83–122. doi: 10.1177/095952874102400109.
  9. Koley B.N., Mukherjee S.R. Spinal preparations and spinal shock. J. Exp. Med. Sci. 1964; 8: 14–24.
  10. Latash M.L., Huang X. Neural control of movement stability: Lessons from studies of neurological patients. Neuroscience. 2015; 301: 39–48. doi: 10.1016/j.neuroscience.2015.05.075.
  11. Sherrington C.S. The integrative action of the nervous system. Second ed. New Haven: Vale Univ. Press. 1947; 440 p.
  12. Eshpai R.A., Grishin S.N., Teplov A.Yu., Safiullin R.S., Morozov G.A., Farhytdinov A.M., Khairullin A.E., Morozov O.G. Simultaneous registration of contraction of diffe­rent types of skeletal muscle in vivo. Izvestiya Samarskogo nauchnogo tsentra Rossiyskoy akademii nauk. 2014; 16 (5-5): 1812–1814. (In Russ.)
  13. Eshpay R.A., Khairullin A.E., Karimova R.G., Nurieva L.R., Rizvanov A.A., Mukhamedyarov M.A., Ziganshin A.U., Grishin S.N. Parameters of single and summated contractions of skeletal muscles in vivo and in vitro. Geny i kletki. 2015; 10 (4): 123–126. (In Russ.)
  14. Moshonkina T.R., Gilerovich E.G., Fedorova E.A., Avelev V.D., Gerasimenko Ju.P., Otellin V.A. Morphofunctional bases of restoration of locomotor movements in rats with complete spinal cord transection. Bjulleten' eks­perimental'noj biologii i mediciny. 2004; (8): 225–229. (In Russ.)
  15. Delbono O. Neural control of aging skeletal ­muscle. Aging Cell. 2003; 2 (1): 21–29. doi: 10.1046/j.1474-9728.2003.00011.x.
  16. Grishin S.N., Ziganshin A.U. Synaptic organization of tonic motor units in vertebrates. Series A: Membrane and Cell Biology. 2015; 9 (1): 13–20. doi: 10.1134/S1990747814060014.
  17. Miledi R., Orkand P. Effect of a fast nerve on slow muscle fibres in the frog. Nature. 1966; 209: 717–718. doi: 10.1038/209717a0.
  18. Radzyukevich T.L. Reinnervation of the mixed muscle of the frog Rana temporaria with a regene­rating homogeneous nerve. J. Evol. Biochem. Physiol 1995; 31 (4): 467–474. (In Russ.)

Supplementary files

Supplementary Files Action
1.
Рис. 1. Влияние спинального шока на параметры сокращения m. extensor digitorum longus (m. EDL) и m. soleus крысы (представлены отдельные репрезентативные треки)

Download (11KB) Indexing metadata

Statistics

Views

Abstract - 47

PDF (Russian) - 3

Cited-By


Article Metrics

Metrics Loading ...

PlumX

Dimensions


© 2021 Valiullin V.V., Khairullin A.E., Eremeev A.A., Teplov A.Y., Shaikhutdinova A.R., Kashtanov N.M., Grishin S.N.

Creative Commons License

This work is licensed
under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.





This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies