Bactericidal capacity of oral neutrophils as a marker for clinical course of inflammatory respiratory diseases in children
- Authors: Pikuza OI1, Fayzullina RA1, Zakirova AM1, Suleymanova ZY.1, Rashitova EL1, Volyanyuk EV1
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Affiliations:
- Kazan State Medical University
- Issue: Vol 101, No 5 (2020)
- Pages: 740-748
- Section: Clinical experiences
- URL: https://kazanmedjournal.ru/kazanmedj/article/view/46499
- DOI: https://doi.org/10.17816/KMJ2020-740
- ID: 46499
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Open Access
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Abstract
Aim. To study the number of neutrophils in the oral cavity, their bactericidal potential, to assess as an indicator for predicting the course of recurrent bronchitis (J40) and community-acquired focal pneumonia in children.
Methods. 87 children between 5 and 10 years old, including 52 children with recurrent bronchitis and 35 with focal community-acquired pneumonia were observed. The control group consisted of 37 conditionally healthy children of a similar age. Viral antigens were studied by chemiluminescence immunoassay. Oral neutrophil counts and functional activity were determined. Antibacterial antibodies were measured by an enzyme-linked immunosorbent assay (ELISA).
Results. 70.11% of patients had a viral antigen, and 57.47% had immunoglobulins M and G against bacterial pathogens. Oral neutrophil counts increased in the main group compared to the control group: up to 163.8±26.5 cells (p <0.001) in recurrent bronchitis, to 110.9±25.5 (p <0.05) in community-acquired pneumonia. By the recovery period, the number of oral neutrophils counts decreased in recurrent bronchitis (1.7 times higher compared to the control group, p <0.01) and remained practically unchanged in community-acquired pneumonia (115.0±26.9, p <0.05). Myeloperoxidase level had opposite changes for the groups compared to the control group: with recurrent bronchitis, it was 1.61±0.09 to the level in the control group (p <0.05), with community-acquired pneumonia — 0.73±0.09 to the level in the control group (p <0.001). The level of lysosomal cationic proteins decreased to 0.77±0.09 to the level in the control group (p <0.05) in recurrent bronchitis, and to 0.80±0.09 (p <0.05) in pneumonia.
Conclusion. In inflammation of the respiratory tract, neutrophil migration to the oral cavity, as well as myeloperoxidase level, increases, indicators of spontaneous luminol-dependent chemiluminescence are activated, and a deficiency of lysosomal cationic proteins occurs; this prevents the penetration of the pathogen into the lower respiratory tract.
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About the authors
O I Pikuza
Kazan State Medical University
Email: azakirova@gmail.com
Russian Federation, Kazan, Russia
R A Fayzullina
Kazan State Medical University
Email: azakirova@gmail.com
Russian Federation, Kazan, Russia
A M Zakirova
Kazan State Medical University
Author for correspondence.
Email: azakirova@gmail.com
Russian Federation, Kazan, Russia
Z Ya Suleymanova
Kazan State Medical University
Email: azakirova@gmail.com
Russian Federation, Kazan, Russia
E L Rashitova
Kazan State Medical University
Email: azakirova@gmail.com
Russian Federation, Kazan, Russia
E V Volyanyuk
Kazan State Medical University
Email: azakirova@gmail.com
Russian Federation, Kazan, Russia
References
- Immunizaciya, vakciny i biologicheskie preparaty. Pnevmokokkovaya infekciya. (Immunization, vaccines and biologicals. Pneumococcal disease.) https://www.who.int/immunization/ru/ (access date: 21.08.2018). (In Russ.)
- Chuchalin A.G., Sinopal'nikov A.I., Kozlov R.S. et al.; Russian Respiratory Society, Interregional association on clinical microbiology and antimicrobial chemotherapy. Clinical guidelines on diagnosis, treatment and prevention of severe community acquired pneumonia in adults. Pulmonologiya. 2014; (4): 13–48. (In Russ.) doi: 10.18093/0869-0189-2014-0-4-13-48.
- Ilenkova N.A., Protasova I.N., Sokolovskaya E.S. Community-acquired pneumonia in children caused by pneumococci of MLSB- and M-phenotype: Clinical cases. Current pediatrics. 2017; 16 (2): 175–179. (In Russ.) doi: 10.15690/vsp.v16i2.1720.
- Zakirova A.M., Pikuza O.I., Shayapova D.T. et al. Effectiveness of nebulizers in the treatment of respiratory tract respiratory infections in children of different ages. Meditsinskiy sovet. 2020; (1): 152–157. (In Russ.) doi: 10.21518/2079-701Х-2020-1-152-157.
- Vavilova V.P., Vavilov A.M., Chercaeva A.H. Prevention of pneumococcal infection in children with chronic diseases of the nasopharynx reduces the incidence of other respiratory tract infections: results of a comparative prospective study. Current pediatrics. 2015; 14 (5): 557–563. (In Russ). doi: 10.15690/vsp. v14i5.1439.
- Baranov A.A., Namazova-Baranova L.S., Vishneva E.A. et al. Primary ciliary dyskinesia in children. Pediatric pharmacology. 2018; 15 (1): 20–31. (In Russ.) doi: 10.15690/pf.v15i1.1840.
- Korovkina E.S., Kostinov M.P. Immune mechanisms of community-acquired pneumonia and copd due to infectious etiology and methods of immunotherapy. Journal of microbiology, epidemiology and immunobiology. 2019; (2): 100–109. (In Russ.) doi: 10.36233/0372-9311-2019-2-100-109.
- Namazova-Baranova L.S., Fedoseenko M.V., Vishneva E.A. et al. Theoretical background and real results: A data review on vaccine prevention of pneumococcal infection in the world. Pediatric pharmacology. 2018; 15 (1): 58–74. (In Russ.) doi: 10.15690/pf.v15i1.1844.
- Suleymanova Z.Ya., Zakirova A.M. Clinical and diagnostic value of indicators of interferon status and antiendotoxin protection in patients with recurrent bronchitis. Diagnostic issues in pediatrics. 2009; 1 (5): 17–19. (In Russ.)
- Andryukov B.G., Bogdanova V.D., Lyapun I.N. Phenotypic heterogeneity of neutrophils: new antimicrobic characteristics and diagnostic technologies. Russian journal of hematology and transfusiology. 2019; 64 (2): 211–221. (In Russ.) doi: 10.35754/0234-5730-2019-64-2-211-221.
- Wang J., Hossain M., Thanabalasuriar A. et al. Visualizing the function and fate of neutrophils in sterile injury and repair. Science. 2017; 358 (359): 111–116. doi: 10.1126/science.aam9690.
- Zakirova A.M., Pikuza O.I., Suleymanova Z.Ya., Kalimullina A.V. The content of oral neutrophils in community-acquired pneumonia and bronchitis in school children. Prakticheskaya Meditsina. 2012; (7-2): 45. (In Russ.)
- Jones H.R., Robb C.T., Perretti M. et al. The role of neutrophils in inflammation resolution. Semin. Immunol. Acad. Press. 2016; 289 (2): 137–145. doi: 10.1016/j.smim.2016.03.007.
- Kaur M., Singh D. Neutrophil chemotaxis caused by chronic obstructive pulmonary disease alveolar macrophages: the role of CXCL8 and the receptors CXCR1/CXCR2. J. Pharmacol. Exp. Therap. 2013; 347 (1): 173–180. DOI: 0.1124/jpet.112.201855.
- Dolgushin I.I., Mezentseva E.A., Savochkina A.Yu. et al. Neutrophil as a multifunctional relay in immune system. Infektsiya i immunitet. 2019; 9 (1): 9–38. (In Russ.) doi: 10.15789/2220-7619-2019-1-9-38.
- Andryukov B.G., Somova L.M., Drobot E.I., Matosova E.V. Defensive strategy of neutrophilic granulocytes against pathogenic bacteria. Zdorov'e, meditsinskaya ekologiya. Nauka. 2017; (1): 4–18. (In Russ.) doi: 10.5281/zenodo.345606.
- Nadzhimitdinov S.T. Osnovnye laboratornye metody issledovaniya morfologii kletok krovi. (Basic laboratory methods for studying the morphology of blood cells.) Tashkent. 1970; 54 р. (In Russ.)
- Slavinskiy A.A., Nikitina G.V. Tsitokhimicheskoe vyyavlenie kationnykh belkov v granulotsitakh krovi amido chernym 10B dlya vizual'noy otsenki i komp'yuternogo analiza izobrazheniya. Klinicheskaya laboratornaya diagnostika. 1999; (2): 35–37 (In Russ.)
- Novikova I.A. Vvedenie v klinicheskuyu laboratornuyu diagnostiku. (Introduction to Clinical Laboratory Diagnostics.) Minsk: Vysheyshaya shkola. 2018; 157–158. (In Russ.)
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