Effect of endocrine disrupting chemicals bisphenol A and triclosan on semen quality

Abstract


Aim. To establish a relationship between the concentration of endocrine disruptors (bisphenol A, triclosan) in testicular tissues or seminal fluid and semen quality in men and experimental animals.

Methods. 53 samples of seminal fluid were investigated from men with normo- and pathozoospermia. In seminal fluid the level of bisphenol A and triclosan were determined by gas chromatography-mass spectrometry. The spermiological study was carried out according to the World Health Organization guidelines (2010). The experimental part of the study was performed on adult male lab Wistar rats (n=28). The amount of spermatozoa per 1 ml of sperm was determined with the calculation of their motility and atypical forms. The concentration of bisphenol A and triclosan in the testicles was measured by gas chromatography-mass spectrometry.

Results. Bisphenol A and triclosan were detected in 100 and 84.9% of the ejaculate samples with a median concentration of 0.15 (0.06–0.31) ng/ml and 0.13 (0.05–0.22) ng/ml, respectively. With the Spearman's rank correlation coefficient, statistically significant correlation was found between the concentration of endocrine disruptors and ejaculate parameters: between bisphenol A and the total count of spermatozoa, their concentration, the proportion of progressively motile spermatozoa, the proportion of spermatozoa with normal morphology and degree of sperm DNA fragmentation. Triclosan concentration correlated with the proportion of spermatozoa with normal morphology and the degree of sperm DNA fragmentation. The experimental part of the study demonstrated that bisphenol A reduced the total number of spermatozoa with an increase in the proportion of atypical forms of spermatozoa and a violation of the proportion of fertile spermatozoa compared to the group of intact animals. Triclosan reduced the proportion of progressively motile spermatozoa.

Conclusion. Bisphenol A and triclosan have a negative effect on sperm quality in men and experimental animals.


S V Chigrinets

South-Ural State Medical University; DNK clinic, Ltd

Author for correspondence.
Email: chigrinstas@gmail.com
Chelyabinsk, Russia; Chelyabinsk, Russia

G V Bryukhin

South-Ural State Medical University

Email: chigrinstas@gmail.com
Chelyabinsk, Russia

  1. Ventimiglia E., Montorsi F., Salonia A. Comorbidities and male infertility: a worrisome picture. Curr. Opin. Urol. 2016; 26 (2): 146–151. doi: 10.1097/MOU.0000000000000259.
  2. Buck Louis G. Male fecundity and its implications for health and disease across the lifespan. Hum. Reprod. 2014; 29 (7): 1351–1352. doi: 10.1093/humrep/deu108.
  3. Eisenberg M., Li Sh., Behr B. et al. Semen quality, infertility and mortality in the USA. Hum. Reprod. 2014; 29 (7): 1567–1574. doi: 10.1093/humrep/deu106.
  4. Korshunov M.N. 33rd Congress of the European Association of urology 2018. Review of papers on the to­pic of “Male infertility”. Daydzhest urologii. 2018; (3): 2–8. (In Russ.)
  5. Nieschlag E., Behre H.M. Andrology: male reproductive health and dysfunction. Berlin, Heidelberg: Springer-­Verlag. 2010; 629 p. doi: 10.1007/978-3-540-78355-8.
  6. Colborn T., von Saal F.S., Soto A.M. Development effects of endocrine-disrupting chemicals in wildlife and humans. Environ. Health Pеrspect. 1993; 101 (5): 378–384. doi: 10.2307/3431890.
  7. Li X., Ying G., Zhao J. et al. 4-Nonylphenol, bisphenol-A and triclosan levels in human urine of children and students in China, and the effects of drinking these bottled materials on the levels. Environ. Intern. 2013; 52: 81–86. doi: 10.1016/j.envint.2011.03.026.
  8. Geens T., Neels H., Covaci A. Distribution of bisphenol-A, triclosan and n-nonylphenol in human adipose tissue, liver and brain. Chemosphere. 2012; 87 (7): 796–802. doi: 10.1016/j.chemosphere.2012.01.002.
  9. WHO laboratory manual for the examination and processing of human semen. World Health Organization, Department of Reproductive Health and Research. 5th ed. Geneva: WHO. 2010; 287 p.
  10. Zholdakova Z.I., Sinitsina O.O., Kharchevnikova N.V. Current state of the issue of bisphenol A toxicity at exposure in doses close to those recognized safe. Toksikologicheskiy vestnik. 2012; (4): 19–25. (In Russ.)
  11. Lan Z., Hyung Kim T., Shun Bi K. et al. Triclosan exhibits a tendency to accumulate in the epididymis and shows sperm toxicity in male sprague-dawley rats. Environ. Toxicol. 2015; 30 (1): 83–91. doi: 10.1002/tox.21897.
  12. Lutskiy D.L., Nikolaev A.A. Morfologicheskoe issledovanie eyakulyata. Metodicheskoe posobie. (Morphological study of ejaculate. Methodological guide.). Astrakhan'. 1999; 46 p. (in Russ.)
  13. Bryukhin G.V., Sizonenko M.L. Characteristic of sperm motility in mature offspring of females with experimental liver diseases of various origins. Bull. Exper. Biol. Med. 2014; 158 (1): 27–29. doi: 10.1007/s10517-014-2683-x.
  14. Wyrobek A.J., Bruce W.R. Chemical induction of sperm abnormalities in mice. Proc. Natl. Acad. Sci. USA. 1975; 72 (11): 4425–4429. doi: 10.1073/pnas.72.11.4425.

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