Identification of Bos taurus and Bos grunniens based on SNP

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The article examined samples of domestic yak and three breeds of cattle to assess the differentiating potential of the polymorphic variants Chr4:68609356G>T (JAZF1 gene), Chr14:35695388G>T (SLCO5A1 gene) and Chr19:63181970C>G (CEP112 gene). The high accuracy (99.67%), specificity (100%) and sensitivity (100%) of the proposed test model consisting of these three polymorphisms for the identification of domestic yaks and cattle were confirmed. A fast and simple identification method has been developed based on this model using competitive allele-specific PCR (KASP) technology, which can significantly reduce the time and financial costs of molecular genetic analysis, as well as reduce the risk of cross-contamination of samples.

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作者简介

V. Kipen

Institute of Genetics and Cytology of the National Academy of Sciences of Belarus

编辑信件的主要联系方式.
Email: v.kipen@igc.by
白俄罗斯, Minsk

Zh. Isakova

Research Institute of Molecular Biology and Medicine; Kyrgyz-Turkish Manas University

Email: v.kipen@igc.by
哈萨克斯坦, Bishkek; Bishkek

M. Patrin

Maxim Medical LLC

Email: v.kipen@igc.by
俄罗斯联邦, Moscow

K. Chekirov

Kyrgyz-Turkish Manas University

Email: v.kipen@igc.by
哈萨克斯坦, Bishkek

K. Aitbaev

Research Institute of Molecular Biology and Medicine

Email: v.kipen@igc.by
哈萨克斯坦, Bishkek

A. Karypova

Kyrgyz-Turkish Manas University

Email: v.kipen@igc.by
哈萨克斯坦, Bishkek

M. Irsaliev

Research Institute of Molecular Biology and Medicine

Email: v.kipen@igc.by
哈萨克斯坦, Bishkek

参考

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2. 1. Graph of allelic discrimination of KASP results for polymorphisms: a – Ch4:68609356G>T (JAZF1); b – Chr14:35695388G>T (SLCO5A1); c – Chr19:63181970C>G (CEP112).

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3. Fig. 2. Graphical representation of a model of three polymorphisms for the identification of domestic yak and cattle: a – 202 samples (samples from Kyrgyzstan); b – 611 samples (samples from Kyrgyzstan and SRA data).

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