Identification of Bos taurus and Bos grunniens based on SNP

Мұқаба

Дәйексөз келтіру

Толық мәтін

Ашық рұқсат Ашық рұқсат
Рұқсат жабық Рұқсат берілді
Рұқсат жабық Тек жазылушылар үшін

Аннотация

The article examined samples of domestic yak and three breeds of cattle to assess the differentiating potential of the polymorphic variants Chr4:68609356G>T (JAZF1 gene), Chr14:35695388G>T (SLCO5A1 gene) and Chr19:63181970C>G (CEP112 gene). The high accuracy (99.67%), specificity (100%) and sensitivity (100%) of the proposed test model consisting of these three polymorphisms for the identification of domestic yaks and cattle were confirmed. A fast and simple identification method has been developed based on this model using competitive allele-specific PCR (KASP) technology, which can significantly reduce the time and financial costs of molecular genetic analysis, as well as reduce the risk of cross-contamination of samples.

Толық мәтін

Рұқсат жабық

Авторлар туралы

V. Kipen

Institute of Genetics and Cytology of the National Academy of Sciences of Belarus

Хат алмасуға жауапты Автор.
Email: v.kipen@igc.by
Белоруссия, Minsk

Zh. Isakova

Research Institute of Molecular Biology and Medicine; Kyrgyz-Turkish Manas University

Email: v.kipen@igc.by
Қазақстан, Bishkek; Bishkek

M. Patrin

Maxim Medical LLC

Email: v.kipen@igc.by
Ресей, Moscow

K. Chekirov

Kyrgyz-Turkish Manas University

Email: v.kipen@igc.by
Қазақстан, Bishkek

K. Aitbaev

Research Institute of Molecular Biology and Medicine

Email: v.kipen@igc.by
Қазақстан, Bishkek

A. Karypova

Kyrgyz-Turkish Manas University

Email: v.kipen@igc.by
Қазақстан, Bishkek

M. Irsaliev

Research Institute of Molecular Biology and Medicine

Email: v.kipen@igc.by
Қазақстан, Bishkek

Әдебиет тізімі

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Әрекет
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Жүктеу
2. 1. Graph of allelic discrimination of KASP results for polymorphisms: a – Ch4:68609356G>T (JAZF1); b – Chr14:35695388G>T (SLCO5A1); c – Chr19:63181970C>G (CEP112).

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Метадеректер
3. Fig. 2. Graphical representation of a model of three polymorphisms for the identification of domestic yak and cattle: a – 202 samples (samples from Kyrgyzstan); b – 611 samples (samples from Kyrgyzstan and SRA data).

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