Vernodalin Triggers ROS-Mediated Apoptosis in TPC-1 Human Papillary Thyroid Cancer Cells via Suppression of the MAPKs Signaling Pathway
- Авторы: Yang X.1, Wei M.2, An Y.3, Liang Q.4, Nan J.3, Vijayalakshmi A.5, Wang Z.3
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Учреждения:
- Department of General Surgery, Xi'an Gaoxin Hospital
- Dialysis Department of Nephrology Hospital, The First Affiliated Hospital of Xian Jiaotong University
- Department of Head and Neck Surgery, Shaanxi Provincial Tumor Hospital, The Affiliated Hospital of Xi'an Jiaotong Univesity
- Department of Head and Neck Surgery, Shaanxi Provincial Tumor Hospital,, The Affiliated Hospital of Xi'an Jiaotong Univesity
- , Galileovasan Offshore and Research And Development Pvt. Ltd
- Выпуск: Том 27, № 14 (2024)
- Страницы: 2151-2158
- Раздел: Chemistry
- URL: https://kazanmedjournal.ru/1386-2073/article/view/644178
- DOI: https://doi.org/10.2174/0113862073286226240220092357
- ID: 644178
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Аннотация
Background:Thyroid Cancer (TC) is an endocrine organ malignancy that has become more common in recent decades. Vernodalin (VN), a cytotoxic sesquiterpene, has been reported to exhibit anticancer properties against human breast and liver cancer cells. However, no study has explored the efficacy of VN with respect to its antiproliferative and apoptotic action on human Papillary Thyroid Cancer cells (PTC).
Objective:The study intended to examine the antitumor and antiproliferative effects of VN and the apoptosis mechanisms underlying its action on TPC-1 human PTC cells.
Methods:In this study, we examined the VN cell viability by MTT assay; performed ROS measurement by DCFH staining method, MMP identification by Rh-123 staining method, and apoptotic morphological assay by employing AO/EB and DAPI stain method, and further, p38 MAPK/ERK/JNK cell proliferation markers were determined by western blotting technique.
Results:The findings showed that VN could inhibit the growth of PTC cells by increasing intracellular ROS, damaging MMP, and stimulating apoptosis in a concentration-dependent manner. The study demonstrated how VN inhibited TPC-1 cell viability by causing ROS-induced cell death via the MAPK signaling pathway.
Conclusion:VN may serve as an agonist to impact apoptosis in PTC cells. In human PTC, VN could play an effective role in chemotherapy. More studies pertaining to animal tumor models are needed to prove its anti-cancer effectiveness in vivo.
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Об авторах
Xijia Yang
Department of General Surgery, Xi'an Gaoxin Hospital
Email: info@benthamscience.net
Meng Wei
Dialysis Department of Nephrology Hospital, The First Affiliated Hospital of Xian Jiaotong University
Email: info@benthamscience.net
Yuan An
Department of Head and Neck Surgery, Shaanxi Provincial Tumor Hospital, The Affiliated Hospital of Xi'an Jiaotong Univesity
Email: info@benthamscience.net
Qinlong Liang
Department of Head and Neck Surgery, Shaanxi Provincial Tumor Hospital,, The Affiliated Hospital of Xi'an Jiaotong Univesity
Email: info@benthamscience.net
Jing Nan
Department of Head and Neck Surgery, Shaanxi Provincial Tumor Hospital, The Affiliated Hospital of Xi'an Jiaotong Univesity
Email: info@benthamscience.net
Annamalai Vijayalakshmi
, Galileovasan Offshore and Research And Development Pvt. Ltd
Email: info@benthamscience.net
Zizhang Wang
Department of Head and Neck Surgery, Shaanxi Provincial Tumor Hospital, The Affiliated Hospital of Xi'an Jiaotong Univesity
Автор, ответственный за переписку.
Email: info@benthamscience.net
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