Thermosensitive TRP-Ion Channels Gene Expression in the Spleen in Normo- and Hypertensive Rats. Effect of the Cold and the Peripheral TRPM8 Ion Channel Activation

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Arterial hypertension leads to changes in the functioning of various organism systems, including the immune system. TRP-ion channels are increasingly attracting attention as targets for the correction of visceral organs, including for therapeutic purposes. The gene expression of the thermosensitive TRP-ion channels (TRPM8, TRPA1, TRPV1, TRPV2, TRPV3 and TRPV4) was studied in the immunocompetent organ, the spleen, using quantitative RT-PCR in normotensive and hypertensive animals under normal conditions, during cooling, and stimulation of the peripheral cold-sensitive ion channel TRPM8. The investigated genes express differently in the spleen of animals of both lines. The expression of cold-sensitive ion channel genes TRPM8 and TRPA1 is reduced in the spleen of hypertensive rats. This is consistent with obtained data on the decrease expression of the TRPM8 ion channel gene in the hypothalamus of hypertensive animals. Deep cooling of the organism, as well as activation of the peripheral (cutaneous) TRPM8-ion channel by menthol, lead to increase expression of the TRPA1 and TRPV1 ion channels genes in the spleen of hypertensive rats, without affecting the expression of genes in normotensive animals. Considering that the ion channels TRPA1 and TRPV1 are involved in inflammation processes, this may indicate a change in the nature of inflammatory reactions in hypertensive animals when organism is exposed to cold. The obtained data expand knowledge about the representation of thermosensitive TRP-ion channels in immunocompetent organs and may indicate a change in the immune status of organism with arterial hypertension.

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A. Evtushenko

Scientific Research Institute of Neurosciences and Medicine

编辑信件的主要联系方式.
Email: evtushenkoaa@neuronm.ru
俄罗斯联邦, Novosibirsk

I. Voronova

Scientific Research Institute of Neurosciences and Medicine

Email: evtushenkoaa@neuronm.ru
俄罗斯联邦, Novosibirsk

T. Kozyreva

Scientific Research Institute of Neurosciences and Medicine

Email: evtushenkoaa@neuronm.ru
俄罗斯联邦, Novosibirsk

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2. Fig. 1. Expression of cold-sensitive ion channel genes TRPA1 and TRPM8 in the spleen of normo- and hypertensive animals. (a) - Photo of PCR product bands of samples and standards for the investigated genes Trpa1, Trpm8 and housekeeping gene Ppia, where I - hypertensive NISAH (ISIAH) line, W - normotensive WAG line. (b) - The mRNA levels of Trpa1 and Trpm8 genes. Gene expression is represented as the number of copies of the investigated gene per 100 copies of the housekeeping gene Ppia. Individual values are represented by triangles in the figure. * - p < 0.05; ** - p < 0.01, significant differences between animals of the studied lines, Student's t-test.

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3. Fig. 2. Effect of slow deep cooling and peripheral TRPM8 ion channel activation by menthol on Trpv1 (a) and Trpa1 (b) gene expression in the spleen of hypertensive rats. Gene expression is presented as the number of copies of the investigated gene per 100 copies of the housekeeping gene Ppia. Individual values are represented by triangles in the figure. * - p < 0.05, significant difference from control group, One-Way ANOVA, post-hoc Fisher test.

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