Eff ect of volatile organic compounds of entomopathogenic fungi of the genus Lecanicillium and their component, the acetic acid, on the female behaviour of the western flower thrips Frankliniella occidentalis (Pergande) (Thysanoptera, Thripidae)

Cover Page

Cite item

Full Text

Open Access Open Access
Restricted Access Access granted
Restricted Access Subscription Access

Abstract

The e ect of VOCs of six entomopathogenic fungal strains from genus Lecanicillium on the behavioral responses of the western ower thrips females Frankliniella occidentalis on vegetative bean plants and in a Y-shaped olfactometer was studied. The signi cant repellent reactions of thrips females and a decrease in a number of o spring by 33-34% were revealed after treatment the beans by conidial suspensions of L. lecanii strains F2 and Vl 29 in the concentration of 1 × 107 spores/ml. Strains Vl 21 ( L. muscarium ) and Vit 71 ( L. attenuatum ), which showed a repellent tendency towards thrips females, also caused a signi cant decrease of o spring number. The strains isolated from aphids (ARSEF 2332 of L. dimorphum and Vl 13 of L. longisporum ) showed a tendency towards attractiveness. In the Y-shaped olfactometer the repellency of the F2 strain and the attractiveness of the ARSEF 2332 strain were con rmed. The in uence of the other studied strains on the thrips behavioral reactions was unreliable. Acetic acid, tested in the olfactometer at doses corresponding to its content in the fungal VOCs over growing mycelium, caused di erent reactions of thrips females depending on the acid concentration: attractive reactions at the dose of 0.17 ppm; neutral - at the dose of 0.34 ppm; and weak repellent - at 0.85 ppm. The obtained data indicate that acetic acid, contained in the fungal VOCs, e ect on the behavioral responses of thrips females. The repellant e ect of the fungal spores of the genus Lecanicillium on thrips females and the negative impact on o spring number increase the e ectiveness of entomopathogenic fungi.

About the authors

G. V Mitina

All-Russian Institute of Plant Protection

Email: galmit@rambler.ru
3, shosse Podbelskogo, St.-Petrsburg, Pushkin,196608

E. A Stepanycheva

All-Russian Institute of Plant Protection

Email: stepanycheva@yandex.ru
3, shosse Podbelskogo, St.-Petrsburg, Pushkin,196608

A. A Choglokova

All-Russian Institute of Plant Protection

Email: 4oglik@inbox.ru
3, shosse Podbelskogo, St.-Petrsburg, Pushkin,196608

M. A Cherepanova

All-Russian Institute of Plant Protection

Email: cherepma@mail.ru
3, shosse Podbelskogo, St.-Petrsburg, Pushkin,196608

References

  1. Кузьмин А. Г., Титов Ю. А., Митина Г. В., Чоглокова А. А. 2021. Масс-cпектометрические исследования состава летучих органических соединений, выделяемых различными видами грибов рода Lecanicillium. Научное приборостроение 31 (4): 71-78. https://doi.org/10.18358/np-31-4-i7178
  2. Митина Г. В., Степанычева Е. А., Петрова М. О. 2019. Влияние летучих соединений и экстрактов мицелия энтомопатогенных грибов на поведенческие реакции и жизнеспособность западного цветочного трипса Frankliniella occidentalis (Pergande). Паразитология 53 (3): 230-240. https://doi.org/10.1134/S0031184719030050
  3. Митина Г. В., Степанычева Е. А., Чоглокова А. А. 2022а. Новые подходы к оценке эффективности энтомопатогенных грибов в микробиологической защите растений от западного цветочного трипса Frankliniella occidentalis (Pergande) (Thysanoptera: Thripidae). Труды Русского энтомологического общества. Санкт-Петербург. Т. 93. Энтомологические исследования в агроэкосистемах, с. 132-137. https://doi.org/10.47640/1605-7678_2022_93_132
  4. Митина Г. В., Степанычева Е. А., Чоглокова А. А., Черепанова М. А. 2022б. Роль летучих органических соединений энтомопатогенных грибов рода Lecanicillium в поведении самок западного цветочного трипса Frankliniella occidentalis (Pergande). В кн.: Сборник статей XVII Международной научно-практической конференции. "Агропромышленный комплекс: состояние, проблемы, перспективы". Пенза: Пензенский государственный аграрный университет, с. 210-213.
  5. Baran B., Krzyżowski M., Cup M., Janiec J., Grabowski M., Francikowski J. 2018. Repellent effect of volatile fatty acids on lesser mealworm (Alphitobius diaperinus). Insects 9 (1): 35. https://doi.org/10.3390/insects9010035
  6. Baverstock J., Roy H. E., Pell J. K. 2009. Entomopathogenic fungi and insect behaviour: From unsuspecting hosts to targeted vectors. BioControl 55 (1): 89-102. https://doi.org/10.1007/s10526-009-9238-5
  7. Bilbo T. R., Kennedy G. G., Walgenbach J. F. 2023. Western flower thrips (Frankliniella occidentalis) field resistance to spinetoram in North Carolina. Crop Protection 165: 106-168. https://doi.org/10.1016/j.cropro.2022.106168
  8. Bojke A., Tkaczuk C., Stepnowski P., Gołębiowski M. 2018. Comparison of volatile compounds released by entomopathogenic fungi. Microbiological Research 214: 129-136. https://doi.org/10.1016/j.micres.2018.06.011
  9. Butt T. M., Coates C. J., Dubovskiy I. M., Ratcliffe N. A. 2016. Entomopathogenic fungi: new insights into host-pathogen interactions. Advances in Genetics 94: 307-364. https://doi.org/10.1016/bs.adgen.2016.01.006
  10. Cao Y., Zhi J. R., Li C., Zhang R. Z., Wang C., Shang B. Z.; Gao Y. L. 2018. Behavioral responses of Frankliniella occidentalis to floral volatiles combined with different background visual cues. Arthropod-Plant Interactions 12: 31-39. https://doi.org/10.1007/s11829-017-9549-x
  11. González-Mas N., Gutiérrez-Sánchez F., Sánchez-Ortiz A., Grandi L., Turlings T. C. J., Manuel Muñoz-Redondo J., Moreno-Rojas J. M., Quesada-Moraga E. 2021. Endophytic colonization by the entomopathogenic fungus Beauveria bassiana affects plant volatile emissions in the presence or absence of chewing and sap-sucking insects. Frontiers in Plant Science 12: 660460. https://doi.org/10.3389/fpls.2021.660460
  12. Hummadi E. H., Dearden A., Generalovic T., Clunie B., Harrott A., Cetin Y., Demirbek M., Khoja S., Eastwood D., Dudley E., Hazir S., Touray M., Ulug D., Gulsen S. H., Cimen H., Butt T. 2021. Volatile organic compounds of Metarhizium brunneum influence the efficacy of entomopathogenic nematodes in insect control. Biological Control 155: 104527. https://doi.org/10.1016/j.biocontrol.2020.104527
  13. Justin G., Robbins P. S., Rocco T. A, Lukasz L. S., Lapointe S. L. 2016. Formic and acetic acids in degradation products of plant volatiles elicit olfactory and behavioral responses from an insect vector. Chemical Senses 41 (4): 325-338. https://doi.org/10.1093/chemse/bjw005
  14. Kepler R. M., Luangsa-Ard J. J., Hywel-Jones N. L., Quandt C. A., Sung G. H., Rehner S. A., Aime M.C., Henkel T. W., Sanjuan T., Zare R., Chen M., Li Z., Rossman A. Y., Spatafora J. W., Shrestha B. 2017. A phylogenetically-based nomenclature for Cordycipitaceae (Hypocreales). IMA Fungus 8 (2): 335-353.https://doi.org/10.5598/imafungus.2017.08.02.08
  15. Kivett J. M., Cloyd R. A., Bello N. M. 2016. Evaluation of entomopathogenic fungi against the western flower thrips (Thysanoptera: Thripidae) under laboratory conditions. Journal of Entomological Science 51 (4): 274-291.https://doi.org/10.18474/JES16-07.1
  16. Lee S. J., Kim S., Kim J. C., Lee M. R., Hossain M. S., Shin T. S., Kim T. H., Kim J. S. 2017. Entomopathogenic Beauveria bassiana granules to control soil-dwelling stage of western flower thrips Frankliniella occidentalis (Thysanoptera: Thripidae). BioControl 62 (5): 639-648. https://doi.org/10.1007/s10526-017-981
  17. Lozano-Soria A., Picciotti U., Lopez-Moya F., Lopez-Cepero J., Porcelli F., Lopez-Llorca L. V. 2020. Volatile organic compounds from entomopathogenic and nematophagous fungi, repel banana black weevil (Cosmopolites sordidus). Insects 11: 509. https://doi.org/10.3390/insects11080509
  18. Mainali B. P., Lim U. T. 2011. Behavioral response of western flower thrips to visual and olfactory cues. Journal of Insect Behavior 24: 436-46. https://doi.org/10.1007/s10905-011-9267-7
  19. Mburu D. M., Maniania N. K., Hassanali A. 2013. Comparison of volatile blends and nucleotide sequences of two Beauveria bassiana isolates of different virulence and repellency towards the termite Macrotermes michaelseni. Journal of Chemical Ecology 39: 101e108. https://doi.org/10.1007/s10886-012-0207-6
  20. Mitina G. V., Stepanycheva E. A., Choglokova A. A., Cherepanova M. A. 2021. Features of behavioral reactions of the peach aphid Myzus persicae (Sulzer, 1776) (Hemiptera, Aphididae) to volatile organic compounds of entomopathogenic fungi of the genus Lecanicillium. Entomological Revue 101 (8): 1015-1023.https://doi.org/10.1134/S0013873821080017
  21. Morath S. U., Hung R., Bennett J. W. 2012. Fungal volatile organic compounds: A review with emphasis on their biotechnological potential. Fungal Biology Reviews 26: 73-83. https://doi.org/10.1016/j.fbr.2012.07.001
  22. Mouden S., Sarmiento K. F., Klinkhamer P. G., Leiss K. A. 2017. Integrated pest management in western flower thrips: past, present and future. Pest Management Science 73 (5): 813-822. https://doi.org/10.1002/ps.4531
  23. Ormond E. L., Thomas A. P. M., Pell J. K., Freeman S. N., Roy H. E. 2011. Avoidance of a generalist entomopathogenic fungus by the ladybird, Coccinella septempunctata. FEMS Microbiology Ecology 77: 229-237.https://doi.org/10.1111/j.1574-6941.2011.01100.x
  24. Pascual-Villalobos M. J., Robledo A. 1998. Screening for anti-insect activity in Mediterranean plants. Industrial Crop and Products 8 (3): 183-194. https://doi.org/10.1016/S0926-6690(98)00002-8
  25. Ponce M. A., Kim T. N., Morrison W. R. III. 2021. A systematic review of the behavioral responses by stored-product arthropods to individual or blends of microbially produced volatile cues. Insects 12: 391. https://doi.org/10.3390/insects12050391
  26. Reitz S. R., Gao Y. L., Kirk W. D. J., Hoddle M. S., Leiss K. A., Funderburk J. E. 2020. Invasion biology, ecology, and management of the western flower thrips. Annual Review of Entomology 65: 17-37.https://doi.org/10.1146/annurev-ento-011019-024947
  27. Rimal S., Sang J., Poudel S., Thakur D., Montell C., Lee Y. 2019. Mechanism of acetic acid gustatory repulsion in Drosophila. Cell Reports 26 (6): 1432-1442.e4. https://doi.org/10.1016/j.celrep.2019.01.042.
  28. Rondot Y., Reineke A. 2017. Association of Beauveria bassiana with grapevine plants deters adult black vine weevils, Otiorhynchus sulcatus. Biocontrol Science and Technology 27: 811-820. https://doi.org/10.1080/09583157.2017.1347604
  29. Skinner M., Gouli S., Frank C. E., Parker B. L., Kim J. S. 2012. Management of Frankliniella occidentalis (Thysanoptera: Thripidae) with granular formulations of entomopathogenic fungi. Biological Control 63: 246-252. https://doi.org/10.1016/j.biocontrol.2012.08.004
  30. Weisskopf L., Schulz S., Garbeva P. 2021. Microbial volatile organic compounds in intra-kingdom and inter-kingdom interactions. Nature Reviews Microbiology 19: 391-404. https://doi.org/10.1038/s41579-020-00508-1
  31. Wu S., Tang L., Fang F., Li D., Yuan X., Lei Z., Gao Y. 2018. Screening, efficacy and mechanisms of microbial control agents against sucking pest insects as thrips. Crop Protection 55: 199-217. https://doi.org/10.1016/bs.aiip.2018.07.005
  32. Zanardi O. Z., Volpe H. X. L, Luvizotto R. A. G., Magnani R. F., Gonzalez F., Carolina C., Oehlschlager C. A., Lehan B. J., Esperança V., Delfno J. Y., Freitas R., de Carvalho R. I., Mulinari T. A., Miranda M. P., Bento J. M. S., Leal W. S. 2019. Laboratory and field evaluation of acetic acid-based lures for male Asian citrus psyllid, Diaphorina citri. Scientific Reports 9: 12920. https://doi.org/10.1038/s41598-019-49469-3
  33. Zhang T., Reitz S. R., Wang H., Lei Z. 2015. Sublethal effects of Beauveria bassiana (Ascomycota: Hypocreales) on life table parameters of Frankliniella occidentalis (Thysanoptera: Thripidae). Journal of Economic Entomology 108 (3): 975-985. https://doi.org/10.1093/jee/tov091
  34. Zhou Y. M., Zou X., Zhi J. R., Xie J. Q., Jiang T. 2020. Fast recognition of Lecanicillium spp., and its virulence against Frankliniella occidentalis. Frontiers in Microbiology 11: 561381. https://doi.org/10.3389/fmicb.2020.56138

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2023 Russian Academy of Sciences