Comparative Analysis of Mesophyll and Bundle Sheath Chloroplasts from Maize Plants Subjected to Salt Stress

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Аннотация

The effect of salt stress was studied in mesophyll (M) and bundle sheath (BS) chloroplasts of maize plants treated with NaCl for 5 days. Pigment content, chlorophyll fluorescence at 77 K, activity of photosystems (PS) I and II, polypeptide compositions and ultrastructure of the thylakoid membranes were determined in plants grown under different salinity conditions (0, 100, 200, and 250 mM NaCl). The salt treatment caused a decrease in fluorescence, photochemical activity of PSII and PSI, as well as the protein content of the thylakoids. At high salt concentrations, the F735/F686 nm fluorescence ratio in M chloroplasts was reduced, while it was stimulated in BS chloroplasts. The photochemical activity of PSII was reduced in both chloroplasts, while there was no statistically significant difference in the activity of PSI compared to the control. According to the analysis of the protein content of the thylakoid membranes of M and BS chloroplasts, polypeptides belonging to the core antenna of PSII (47 kDa and 43 kDa) and LHCII (28-24 kDa) were present in both types of membranes, but their intensity was weak in BS thylakoids. The synthesis of the 68 kDa apoprotein belonging to the core of PSI was inhibited in the M membranes. There was no noticeable change in the membrane system of BS thylakoids. Salt stress had a greater impact on the ultrastructure of M chloroplasts than on BS ones and caused the formation of granal stacking in BS chloroplast. These results may indicate different responses of the two chloroplast types to salt stress.

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Авторлар туралы

N. Alieva

Institute of Molecular Biology & Biotechnologies

Хат алмасуға жауапты Автор.
Email: irada.huseynova@science.az
Әзірбайжан, Baku

D. Alieva

Institute of Molecular Biology & Biotechnologies

Email: irada.huseynova@science.az
Әзірбайжан, Baku

S. Suleymanov

Institute of Molecular Biology & Biotechnologies

Email: irada.huseynova@science.az
Әзірбайжан, Baku

F. Rzayev

Azerbaijan Medical University

Email: irada.huseynova@science.az
Әзірбайжан, Baku

E. Gasimov

Azerbaijan Medical University

Email: irada.huseynova@science.az
Әзірбайжан, Baku

I. Huseynova

Institute of Molecular Biology & Biotechnologies

Email: irada.huseynova@science.az
Әзірбайжан, Baku

Әдебиет тізімі

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2. Fig. 1. Effect of salt stress on chlorophyll content and Chl a/b ratio in thylakoids of M and OB maize leaves. Significance levels: * p < 0.05; ** p < 0.01; *** p < 0.001. p-values were calculated using the t-test calculator for two independent averages under the two-tailed hypothesis

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3. Fig. 2. Electrophoresis of M and OB thylakoids of maize plants on a 10-25% PAG gradient in the presence of 0.1% SDS. a - Electrophoresis of M (lanes 1-4) and OB (lanes 5-8) thylakoids in a 10-25% gradient gel in the presence of 0.1% SDS: 1 and 5 - 0 mM NaCl; 2 and 6 - 100 mM NaCl; 3 and 7 - 200 mM NaCl; 4 and 8 - 250 mM NaCl; M - protein markers. b - Densitogram of M thylakoids electrophoresis; curves 1-4 correspond to M thylakoids from plants treated with 0, 100, 200, and 250 mM NaCl, respectively

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4. Fig. 3. Effect of salt stress on fluorescence spectra (at 77 K) of chloroplasts of mesophyll (a) and cladding (b) chloroplasts

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5. Fig. 4. Ultrastructure of chloroplasts of mesophyll cells in the middle part of mature second leaf plates of Zea mays after NaCl treatment for five days. a - Chloroplasts of control plant (0 mM NaCl) with well-organised facets. b, c, d - Chloroplasts of plants treated with 100 mM, 200 mM and 250 mM NaCl, respectively. Cw - cell wall, P - plastoglobules, G - granule thylakoid membranes, L - lamellae

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6. Fig. 5. Ultrastructure of chloroplast chloroplasts of OB cells in the middle part of mature second leaf plates of Zea mays after NaCl treatment for five days. a - Chloroplasts of control plant (0 mM NaCl). b, c, d - Chloroplasts of plants treated with 100 mM, 200 mM and 250 mM NaCl, respectively. Cw - cell wall, P - plastoglobules, black arrows indicate the formation of facets

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