Morphological and molecular identification of tissue nematode Philometroides strelkovi (Chromadorea: Dracunculoidea) from three cyprinid species in the North of Primorsky Region

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Abstract

The paper presents a redescription of the parasitic nematode Philometroides strelkovi (Chromadorea: Dracunculoidea) based on the new morphometric data. New hosts from the Cyprinidae family (Rhodeus sericeus, Hemiculter leucisculus, Hemibarbus labeo) and locality – the Luchegorsk reservoir belonging to the Amur River basin (Pozharsky district of Primorsky Krai in the south of the Russian Far East) have been described. For the first time, sequences of the 18S rRNA marker gene have been obtained for P. strelkovi. On the basis of genetic data, we performed phylogenetic reconstruction and showed the clustering of P. strelkovi, separate from the sister species P. moraveci, confirmed by high statistical support at branching nodes and the size of genetic distances — 0.11–0.56% between P. strelkovi and other representatives of the genus, along with intergeneric divergence of >4%. In the light of new molecular data, the question of the artificiality of the genera Philometroides and Philometra is raised.

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About the authors

K. S. Vainutis

Zhirmunsky National Scientific Center of Marine Biology, Far Eastern Branch of the Russian Academy of Sciences; The Far Eastern State Technical Fisheries University (FESTFU)

Author for correspondence.
Email: vainutisk@gmail.com
Russian Federation, Vladivostok 690041, 17 Palchevskogo Street; 52B Lugovaya Street, Vladivostok 690087

A. N. Voronova

Pacific branch of the Federal State Budget Scientific Institution “Russian Federal Research Institute of Fisheries and oceanography”

Email: vainutisk@gmail.com
Russian Federation, 4 Alley Shevchenko, Vladivostok 690091

M. E. Andreev

cPacific branch of the Federal State Budget Scientific Institution “Russian Federal Research Institute of Fisheries and oceanography”; Far Eastern Federal University

Email: vainutisk@gmail.com
Russian Federation, 4 Alley Shevchenko, Vladivostok 690091; 10 Ajax Bay, Russky Island, Vladivostok 690922

N. E. Zyumchenko

Far Eastern Federal University

Email: vainutisk@gmail.com
Russian Federation, 10 Ajax Bay, Russky Island, Vladivostok 690922

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2. Fig. 1. Common bitterling infected with P. strelkovi, localization of the nematode is indicated by black arrows (a), anterior part of the female body (b), photograph of the anterior end of the female body (c), posterior end of the female body (g); photograph of the posterior end of the female body (d): b – bulbus, cp – cloacal papillae, dg – dorsal gland, es – esophagus, in – intestine, n – nerve ring, op – oral papillae, ov – ovary, ut – uterus.

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3. Fig. 2. Phylogenetic tree for representatives of the family Philometridae based on 1820 bp 18S rDNA sequences. Node support is BI posterior probabilities. Fish species described as new hosts for P. strelkovi and the type hosts of P. pseudaspii (syn. P. ganzhounensis) and P. moraveci are shown. Key groups are highlighted in color.

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