Rhodococcus qingshengii GlMm1 as the Basis of a Biosensor for Determination of the Fungicide Carbendazim

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The possible application of Rhоdococcus qingshengii strain GlMm1, isolated from a Dead Sea clay sample, as the basis of a biosensor for determining the benzimidazole fungicide carbendazim was investigated. High sensitivity of the biosensor under neutral pH and up to 500 mM NaCl at 2 to 160 μM carbendazim was maintained for up to 30 days.

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作者简介

T. Kuvichkina

G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Federal Research Center “Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences”

Email: lenokap80@gmail.com
俄罗斯联邦, Pushchino, 142290

E. Kaparullina

G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Federal Research Center “Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences”

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Email: lenokap80@gmail.com
俄罗斯联邦, Pushchino, 142290

N. Doronina

G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Federal Research Center “Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences”

Email: lenokap80@gmail.com
俄罗斯联邦, Pushchino, 142290

A. Reshetilov

G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Federal Research Center “Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences”

Email: lenokap80@gmail.com
俄罗斯联邦, Pushchino, 142290

参考

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2. Fig. 1. Calibration dependence of the biosensor based on the BcI of Rhodococcus qingshengii GlMm1 on the concentration of carbendazim. Biosensor response in picoamps per second (pA/s).

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3. Fig. 2. Substrate specificity of the BcI strain Rh. qingshengii GlMm1. Designations: 1 — catechol; 2 — fructose; 3 — L(‒) ‒ arabite; 4 — D-glucose; 5 — D-sorbitol; 6 ‒ D(+)-cellobiose; 7 — methylamine; 8 — dimethylamine; 9 ‒ carbendazim (substrate concentration 0.1 mM). Biosensor response in picoamps per second (pA/s).

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