Relationship between phytochrome photoactivity and the degree of auxin binding to the receptor in cotton genotypes differing in wilt tolerance

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Abstract

The paper studies some characteristics of three groups of cotton varieties that differ in their resistance to wilt: non-resistant, moderately resistant and resistant. The activity of the phytochrome system in etiolated cotton seedlings was studied using the low-temperature luminescence method, and the ability of red light to increase the specific binding of auxin to its binding sites was studied using the method of studying the binding of radioactively labeled auxin 3H-IAA to auxin-binding sites in membrane preparations and soluble proteins from cotton hypocotyls. It has been shown that the higher the resistance to wilt in different cotton varieties, the higher their activity of the phytochrome system and the higher the increase under the influence of red light, the specific binding of 3H-IAA with membrane preparations and soluble proteins obtained from these cotton varieties. Establishing a direct correlation between cotton resistance to wilt, phytochrome photoactivity and the level of auxin affinity for its receptor creates prospects for further progress in the study of phytochrome-phytohormonal interactions in the regulation of cotton plant resistance to Verticillium dahliae.

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About the authors

I. G. Akhmedzhanov

National University of Uzbekistan

Author for correspondence.
Email: iskakhm@mail.ru
Uzbekistan, Tashkent, 100047

А. K. Tonkikh

Institute of Microbiology of the Academy of Sciences of the Republic of Uzbekistan

Email: anatoliytonkikh@mail.ru
Uzbekistan, Tashkent, 100047

M. M. Khotamov

Institute of Genetics and Plant Experimental Biology of the Academy of Sciences of the Republic of Uzbekistan

Email: mansurhatamov@mail.ru
Uzbekistan, Yukori-Yuz, 111208

P. G. Merzlyak

National University of Uzbekistan

Email: galores@list.ru
Uzbekistan, Tashkent, 100047

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2. Fig. 1. Excitation spectra of low-temperature luminescence of etiolated cotton seedlings before irradiation (1), after irradiation with far-red (2) and red light (3).

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