Preparation, Crystallization, and Preliminary X-ray Diffraction Analysis of Recombinant House Dust Mite Allergen Der p 3 from Dermatophagoides pteronyssinus

V. I. Timofeev; Тимофеев В. И.; Yu. A. Abramchik; Абрамчик Ю. А.; N. E. Zhukhlistova; Жухлистова Н. Е.; O. O. Mikheeva; Михеева О. О.; M. B. Shevtsov; Шевцов М. Б.; E. A. Zayats; Заяц Е. А.; D. D. Lykoshin; Лыкошин Д. Д.; M. A. Kostromina; Костромина М. А.; R. S. Esipov; Есипов Р. С.; I. P. Kuranova; Куранова И. П.

doi:10.31857/S0023476122060273

Preparation, Crystallization, and Preliminary X-ray Diffraction Analysis of Recombinant House Dust Mite Allergen Der p 3 from Dermatophagoides pteronyssinus

Autores: Timofeev V.I.¹^,2, Abramchik Y.A.¹^,3, Zhukhlistova N.E.¹, Mikheeva O.O.³, Shevtsov M.B.⁴, Zayats E.A.³, Lykoshin D.D.³, Kostromina M.A.³, Esipov R.S.³, Kuranova I.P.¹^,2
Afiliações:
1. Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics,” Russian Academy of Sciences, 119333, Moscow, Russia
2. National Research Centre “Kurchatov Institute,”, 123098, Moscow, Russia
3. Shemyakin−Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997, Moscow, Russia
4. Moscow Institute of Physics and Technology, 141701, Dolgoprudny, Moscow oblast, Russia
Edição: Volume 68, Nº 1 (2023)
Páginas: 46-50
Seção: STRUCTURE OF MACROMOLECULAR COMPOUNDS
URL: https://kazanmedjournal.ru/0023-4761/article/view/673539
DOI: https://doi.org/10.31857/S0023476122060273
EDN: https://elibrary.ru/DMUZXL
ID: 673539

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Resumo

The high-producing strain С3029/pGro7/pERDerp3 for the house dust mite allergen Der p 3 from Dermatophagoides pteronyssinus, expressing the recombinant protein in Escherichia coli in the soluble form, was constructed. A procedure was developed for the purification of the recombinant allergen. Crystals of the recombinant protein Der p 3 suitable for X-ray diffraction analysis were grown by the vapor-diffusion method. The X-ray diffraction data set was collected to 2.25 Å resolution at the European Synchrotron Radiation Facility (ESRF, France, ID23-1 beamline) at 100 K. The crystals belong to sp. gr. С121 and contain two enzyme molecules per asymmetric unit.

Palavras-chave

STRUCTURE OF MACROMOLECULAR COMPOUNDS

Sobre autores

V. Timofeev

Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics,” Russian Academy of Sciences, 119333, Moscow, Russia; National Research Centre “Kurchatov Institute,”, 123098, Moscow, Russia

Email: inna@crys.ras.ru
Россия, Москва; Россия, Москва

Yu. Abramchik

Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics,” Russian Academy of Sciences, 119333, Moscow, Russia; Shemyakin−Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997, Moscow, Russia

Email: inna@crys.ras.ru
Россия, Москва; Россия, Москва

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