Characteristics of the complex of polyphenol compounds of Yakutian endemic  Dracocephalum jacutense  Peschkova using tandem mass spectrometry

Zh. M. Okhlopkova; Охлопкова Ж. М.; M. P. Razgonova; Разгонова М. П.; P. S. Egorova; Егорова П. С.; K. S. Golokhvast; Голохваст К. С.

doi:10.31857/S0015330322600723

Characteristics of the complex of polyphenol compounds of Yakutian endemic Dracocephalum jacutense Peschkova using tandem mass spectrometry

Authors: Okhlopkova Z.M.¹, Razgonova M.P.²^,3, Egorova P.S.⁴, Golokhvast K.S.⁵^,3^,6
Affiliations:
1. North-Eastern Federal University
2. Far Eastern Federal University, Advanced Engineering School, Institute of Biotechnology, Bioengineering, and Food Systems
3. Federal Research Center Vavilov All-Russia Institute of Plant Genetic Resources (VIR)
4. Institute for Biological Problems of Permafrost, Siberian Branch, Russian Academy of Sciences
5. Far Eastern Federal University, REC Nanotechnology, Polytechnic Institute
6. Siberian Federal Scientific Center for Agrobiotechnologies, Russian Academy of Sciences
Issue: Vol 70, No 3 (2023)
Pages: 327-336
Section: ЭКСПЕРИМЕНТАЛЬНЫЕ СТАТЬИ
URL: https://kazanmedjournal.ru/0015-3303/article/view/648180
DOI: https://doi.org/10.31857/S0015330322600723
EDN: https://elibrary.ru/IBPENP
ID: 648180

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Abstract

Dracocephalum jacutense Peschkova is a rare and endangered species of the genus Dracocephalum L. of Lamiaceae family. The species was first described in 1997 and listed in the Red Data Book of Yakutia (2017). Until now, due to the rare occurrence of D. jacutense, almost none of its phytochemical studies have been carried out and there is no information on the content of biologically active compounds (secondary metabolites) of the plant. In this work, using the method of tandem mass spectrometry, a complex of polyphenolic compounds was studied in the inflorescences of the Dracocephalum jacutense, collected in the areas of its first discovery - the Kobyaysky district of the Republic of Sakha (Yakutia). As a result of the analysis, 48 polyphenolic compounds were found in the alcohol extracts of Dracocephalum jacutense inflorescences, among which 17 flavones, 7 flavonols, 3 flavan-3-ols, 12 phenolic acids, 1 lignan, 2 coumarins, 6 anthocyanidins were identified. It should be noted that more than half (30 compounds) of all the identified polyphenols for representatives of the genus Dracocephalum L. were discovered for the first time. The results obtained state the unique composition of polyphenolic compounds in the inflorescences of D. jacutense Peschkova. In order to preserve this sparse local endemic of Eastern Siberia, work has begun on the development of an effective method for its micropropagation in vitro.

Keywords

Dracocephalum jacutense, HPLC-MS/MS, tandem mass spectrometry, polyphenolic compounds, CID spectrum

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