Kazan medical journalKazan medical journal0368-48142587-9359Eco-Vector780910.17816/KMJ2018-053Research ArticleMorphological characteristics of experimental ischemic wound healing after application of autoand hetero-fibroblasts and dermal equivalentShapovalovaE YuShapovalova_L@mail.ruBoykoT AShapovalova_L@mail.ruBaranovskiyYu GShapovalova_L@mail.ruLuginI AShapovalova_L@mail.ruMedical Academy named after S.I. Georgievsky, V.I. Vernadsky Crimean Federal University15022018991535906022018Copyright © 2018, Shapovalova E.Y., Boyko T.A., Baranovskiy Y.G., Lugin I.A.2018<p><strong>Aim.</strong> To study morphological structure, collagen synthesis and angiogenesis in biopsy samples of newly formed epidermis and derm on day 19 of their repair in experimental ischemic wound after application of auto- and hetero-fibroblasts as well as after transplantation of dermal equivalent with hetero-fibroblasts.</p>
<p><strong>Methods.</strong> The study was performed on 28 mature C57/B1 white mice younger than 1 year. Around and into the bed of surgical skin wound in the scapular region, 0.4 ml of suspension of fibroblasts and 1.33 million cells of dermal equivalent were injected. Biopsy sample was embedded in paraffin and was stained with hematoxylin and eosin and by Weigert-Van Gieson.</p>
<p><strong>Results.</strong> Most importantly, regenerative histogenesis is affected by introduction of auto-fibroblasts, when the greatest thickness of the epidermis is registered and angiogenesis and collagen formation are most active. At the same time, the most differentiated epidermis is observed after transplantation into the wound of dermal equivalent with hetero-bifroblasts.</p>
<p><strong>Conclusion.</strong> The beneficial effect of dermal equivalent with hetero-bifroblasts differs from the effect of auto-fibroblast suspension by only a few percent: epidermal thickness by 4.29%, the area of collagen fibers by 2.66%, the area of blood vessels by 4.04%, that makes such differences unreliable.</p>regenerative histogenesisdermal equivalentfibroblastskinangiogenesisрегенераторный гистогенездермальный эквивалентфибробласткожаангиогенез[Govrin G. New method for treating hard-to-heal wounds: clinical experience with charged polystyrene microsphers. Wounds. 2010; 6 (4): 52–61.][Danilov R.K. Ranevoy protsess: gistogeneticheskie osnovy. (Wound process: histogenetic basis.) Saint Petersburg: VMedA. 2008; 380 p. (In Russ.)][Khrupkin V.I., Zubritskiy V.F., Ivashkin A.N. et al. Dermatoplastika ranevykh defektov. (Dermatoplasty of wound defects.) Moscow: GEOTAR-Media. 2009; 102 p. (In Russ.)][Vinnik Yu.S., Salmina A.B., Drobushevskaya A.I. et al. Cellular technologies and tissue engineering in the treatment of long-term healing wounds. Vestnik eksperimental’noy i klinicheskoy khirurgii. 2011; 4 (2): 392–397. (In Russ.)][Shapovalova E.Yu., Boyko T.A., Baranovskiy Yu.G. et al. Morphological characteristics of the healing of an ischemic experimental wound on the 12th day after application of auto- and hetero-fibroblasts and dermal equivalent. Mezhdunarodnyy nauchno-issledovatel’skiy zhurnal. 2017; (8-3): 51–55. (In Russ.)][Porter S. The role of the fibroblast in wound contraction and healing. Wounds. 2007; 3 (2): 45–48.][Zorin V.L., Zorina A.I., Petrakova O.S., Cherkasov V.R. Dermal fibroblasts for the treatment of skin defects. Geny & Kletki. 2009; 4 (4): 26–40. (In Russ.)][Meleshina A.V., Bystrova A.S., Rogovaya O.S. et al. Tissue-engineered skin constructs and application of stem cells for creation of skin equivalents (review). Sovremennye tekhnologii v meditsine. 2017; 9 (1): 198–218. (In Russ.) DOI: 10.17691/stm2017.9.1.24.][Baranovskiy Yu.G., Il’chenko F.N., Shapovalova E.Yu. Method for modeling trophic ulcers in laboratory mice in the experimental model. Vestnik neotlozhnoy i vosstanovitel’noyk hirurgii. 2016; 1 (2): 258–260. (In Russ.)][Andreev D.Yu., Abramova N.V., Blinova M.I., Pinaev G.P. Effectiveness of cutaneous plastics and dermal equivalent in the treatment of extensive ulcers of the tibia of mixed origin. Vestnik khirurgii im. I.I. Grekova. 2013; 172 (1): 104–107. (In Russ.)]