Kazan medical journalKazan medical journal0368-48142587-9359Eco-Vector203110.17750/KMJ2015-802Research ArticleEffect of chondroitin sulfate on the type I collagen metabolism in the compact bone in alloxan-induced ratsVyatkinV Avyatkinva@yandex.ruButolinE Gvyatkinva@yandex.ruIvanovV Gvyatkinva@yandex.ruIzhevsk State Medical Academy, Izhevsk, Russia1510201596580280628032016Copyright © 2015, Vyatkin V.A., Butolin E.G., Ivanov V.G.2015<p><strong>Aim.</strong> To study the type I collagen metabolism in the compact bone in rats with alloxan-induced diabetes receiving sulfated glycosaminoglycans.</p>
<p><strong>Methods.</strong> The study was performed on 67 white outbred male rats with body weight of 180-220 g. Mortality at diabetes reproduction was 44.8%. To clarify the role of exogenous glycosaminoglycans on bone collagen metabolism at diabetes mellitus, 16 animals with alloxan-induced diabetes received 1 mg/kg of chondroitin sulfate intramuscularly every second day. The second group (21 animals) with alloxan-induced diabetes did not received any chondroitin sulfate. Control group included 10 intact animals who were administered a single injection on 0.5% ml of normal saline. The levels of type I collagen metabolism markers (PINP - aminoterminal propeptide of type I procollagen, a marker of bone formation; β-CrossLaps - β-isomerized carboxy-terminal cross-linking region of type I collagen, a marker of bone resorption) and the amount of total collagen were determined in homogenates of femoral shaft.</p>
<p><strong>Results.</strong> Administration of alloxan to the animals has induced the development of diabetes mellitus. The levels of PINP and β-CrossLaps was significantly higher in alloxan-induced rats which were administered chondroitin sulfate compared to rats with «isolated» alloxan-induced diabetes by 21 (p=0.001) and 28 (p=0.01) days of follow-up, the level of total collagen was higher at 70% at 28 day of the experiment (p=0.0004).</p>
<p><strong>Conclusion.</strong> Effect of sulfated glycosaminoglycans on type I collagen metabolism of the compact bone in animals with «isolated» alloxan-induced diabetes is manifested by intensified catabolic and anabolic processes with a predominance of the latter over the control and alloxan-induced rats at 21 and 28 days of the experiment.</p>PINPdiabetic osteopathytype I collagenβ-CrossLapsPINPchondroitin sulfatealloxan diabetesдиабетическая остеопатияколлаген I типаβ-СrossLapsхондроитина сульфаталлоксановый диабет[Пальчикова Н.А., Селятицкая В.Г., Шорин Ю.П. Количественная оценка чувствительности экспериментальных животных к диабетогенному действию аллоксана // Пробл. эндокринол. - 1987. - №4. - С. 65-68. [Pal’chikova N.A., Selyatitskaya V.G., Shorin Yu.P. Quantitative assessment of the sensitivity of experimental animals to diabetes-inducing action of alloxan. Problemy endokrinologii. 1987; 4: 65-68. (In Russ.)]][Сахарный диабет: острые и хронические осложнения / Под ред. И.И. Дедова, М.В. Шестаковой. - М.: МИА, 2011. - 480 с. [Sakharnyy diabet: ostrye i khronicheskie oslozhneniya. (Diabetes mellitus: acute and chronic complications.) Ed. by I.I. Dedov, M.V. Shestakova. Moscow: MIA. 2011; 480 p. (In Russ.)]][Черкасова О.П., Кузнецова Н.В., Пальчикова Н.А., Селятицкая В.Г. Активность адренокортикальной системы при экспериментальном диабете у крыс // Сахар. диабет. - 2011. - №2. - С. 37-40. [Cherkasova O.P., Kuznetsova N.V., Palchikova N.A., Selyatitskaya V.G. Activity of the adrenocortical system in rats with experimental diabetes. Sakharnyy diabet. 2011; 2: 37-40. (In Russ.)]][Шараев П.Н., Богданов Н.Г., Ямолдинов Р.Н. Об обмене коллагена в коже при различной обеспеченности организма витамином K // Бюлл. эксперим. биол. и мед. - 1976. - Т. 81, №6. - С. 665-666. [Sharaev P.N., Bogdanov N.G., Yamoldinov R.N. About the collagen metabolism in skin and vitamin K status. Byulleten’ eksperimental’noy biologii i meditsiny. 1976; 81 (6): 665-666. (In Russ.)]][Abdulameer S.A., Syed Sulaimman S.A., Hassali M.A. et al. Osteoporosis and type 2 diabetes mellitus: what do we know, and what we can do? // Patient Prefer Adherence. - 2012. - Vol. 6. - P. 435-448. http://dx.doi.org/102147/PPA.S32745.][Abuohashish H.M., Al-Rejaie S.S., Al-Hosaini K.A. et al. Alleviating effects of morin against experimentally-induced diabetic osteopenia // Diab. & Metab. Syndrome. - 2013. - Vol. 5. - Р. 5. http://dx.doi.org/10.1186/1758-5996-5-5.][Briot K., Roux C. Glucocorticoid-induced osteoporosis // RMD Open. - 2015. - Vol. 1. - Р. 1. - DOI: http://dx.doi.org/10.1136/rmdopen-2014-000014.][Campo G.M., Avenoso A., Campo S. et al. Efficacy of treatment with glycosaminoglycans on experimental collagen-induced arthritis in rats // Arthritis Res. Ther. - 2003. - Vol. 5, N 3. - P. R122-R131. http://dx.doi.org/10.1186/ar748.][Iovu M., Dumais G., du Souich P. Anti-inflammatory activity of chondroitin sulfate // Osteoarthr. Cartil. - 2008. - Vol. 16, suppl. 2. - P. S14-S18. http://dx.doi.org/10.1016/j.joca.2008.06.008.][Krege J.H., Lane N.E., Harris J.M. et al. PINP as a biological response marker during teriparatide treatment for osteoporosis // Osteoporos. Int. - 2014. - Vol. 25, N 9. - P. 2159-2171. http://dx.doi.org/10.1007/s00198-014-2646-0.][Li M., Li Y., Zhang Z. Chinese Bone Turnover Marker Study: reference ranges for C-terminal telopeptide of type I collagen and procollagen I N-terminal peptide by age and gender // PLoS ONE. - 2014. - Vol. 9. - Р. 8. http:/dx.doi.org/10.1371/journal.pone.0103841.][Parisuthiman D., Mochida Y., Duarte W.R. et al. Biglycan modulates osteoblast differentiation and matrix mineralization // J. Bone Miner. Res. - 2005. - Vol. 20, N 10. - P. 1878-1886. http://dx.doi.org/10.1359/JBMR.050612.][Principles of bone biology. 2nd ed. / Ed. J.P. Bilezikian, L.G. Raisz, G.A. Rodan. - Academic Press, 2002. - 1696 p.][Steplewsky A., Fertala A. Inhibition of collagen fibril formation // Fibr. Tissue Rep. - 2012. - Vol. 5. http://dx.doi.org/10.1186/1755-1536-5-S1-S29.]