Kazan medical journalKazan medical journal0368-48142587-9359Eco-Vector193410.17816/KMJ1934Research ArticleThe characteristics of serum circulating immune complexes of patients with atopic asthma with different severity degreeSkiboY Vyuliya_ksu@mail.ruKurmaevaN S-TsibulkinaV N-MustafinI G-AbramovaZ I-Kazan (Volga Region) Federal University, Kazan, RussiaKazan State Medical University, Kazan, Russia1510201394574474828032016Copyright © 2013, Skibo Y.V., Kurmaeva N.S., Tsibulkina V.N., Mustafin I.G., Abramova Z.I.2013Aim. To evaluate the serum level of pathogenic circulating immune complexes in patients with mild and severe atopic bronchial asthma. Methods. Serum samples of patients with atopic asthma of mild persistent (30 patients) and severe persistent (20 patients) forms were analyzed. The control group consisted of 15 healthy volunteers. To detect the giant, large, medium and small-sized serum immune complexes, 3, 3.5, 4 and 7% polyethyleneglycol-6000 solutions were used. For quantitative evaluation of the immune complexes we measured the ultraviolet optical density at 280 nm wave length. To separate the immune complexes from immunoglobulin, Protein-G-Sepharose was used. Determination of the protein composition of circulating immune complexes was performed by electrophoresis in 8% polyacrylamide gel. Results. The concentration of immune complexes was increased in patients with bronchial asthma compared to healthy donors. Small and medium-sized immune complexes were prevailing, their concentrations correlated with the severity of asthma. Large, medium and small-sized immune complexes participated in immunopathological reactions in patients with both mild and severe asthma, with immune complexes pathogenicity coefficient significantly increased depending on the severity of the disease. Electrophoretic analysis of circulating immune complexes has shown the presence of proteins with molecular weight of 60 kDa in the complexes of all sizes. In the severe asthma group, an antigen fraction with a molecular mass of 36 kDa within the small-sized molecular complexes was revealed. Conclusion. The observed increase of small and medium-sized circulating immune complexes serum levels in patients with bronchial asthma may be an indicator of of these patients predisposal to autoimmune reactions development.circulating immune complexespathogenicity coefficientbronchial asthmaatopyautoimmunityциркулирующие иммунные комплексыкоэффициент патогенностибронхиальная астмаатопияаутоиммунитет[Рудык Б.И., Барановский П.В. Сравнительная оценка изучения содержания иммунных комплексов при инфаркте миокарда, бронхиальной астме и ревматоидном артрите // Тер. арх. - 1984. - №10. - С. 17-19.][Стручков П.В., Константинова Н.А., Лаврентьев В.В., Чучалин А.Г. Скрининг-тест для оценки патогенных свойств циркулирующих иммунных комплексов // Лаб. дело. - 1985. - Т. 7. - С. 410-412.][Чучалин А.Г. Глобальная стратегия лечения и профилактики бронхиальной астмы. - М.: Атмосфера, 2008. - 108 с.][Bakunts G., Arakelyan A., Boyajyan A., Poghosyan A. Antigen composition of circulating immune complexes in the blood of patients with hemorrhagic strokes // Westnik IAELPS. - 2001. - Vol. 6,N 42. - P. 97-99.][Buc M., Dzurilla M., Bucova M. Immunophathogenesis of bronchial asthma //Arch. Immunol. Ther. Exp. - 2009. - Vol. 57. - P. 331-344.][Cengic M., Rasic S. Importance of determination of circulating immune complexes in systemic lupus erythematosus // Med. Arch. - 2002. - Vol. 56. - P. 267-270.][Mackay I.R., Water J.V., Gershwin M.E. Autoimmunity: thoughts for the millennium // Clin. Rev. Allergy Immunol. - 2000. - Vol. 18,N 1. - P. 87-117.][Nangaku M., Couser W.G. Mechanisms of immune-deposit formation and the mediation of immune renal injury // Clin. Exper. Nephrol. - 2005. - Vol. 9. - P. 183-191.][Nezlin R.A. Quantitative approach to the determination of antigen in immune complexes // J. Immunol. Methods. - 2000. - Vol. 237. - P. 1-16.][Rottem M., Shoenfeld Y. Asthma as a paradigm for autoimmune disease // Int. Arch. Allergy Immunol. - 2003. - Vol. 132,N 3. - P. 210-214.][Voskuyl A.E., Hazes J.M., Zwinderman A.H. et al. Diagnostic strategy for the assessment of rheumatoid vasculitis // Ann. Rheum. Dis. - 2003. - Vol. 62,N 5. - P. 407-413.][Ye Y.M., Nahm D.H., Kim S.H. et al. Circulating autoantibodies in patients with aspirin-intolerant asthma: an epiphenomenon related to airway inflammation // J. Korean Med. Sci. - 2006. - Vol. 21. - P. 412-417.]