Kazan medical journalKazan medical journal0368-48142587-9359Eco-Vector161110.17750/KMJ2015-905Research ArticlePatterns of endothelium-dependent circulation reactions development in patients with ischemic stroke in case of oxigenation disturbancesRyabchenkoA Yunevrolog2007@inbox.ruDolgovA Mnevrolog2007@inbox.ruDenisovE Nnevrolog2007@inbox.ruRussanovaN Rnevrolog2007@inbox.ruGumanovaN Gnevrolog2007@inbox.ruOrenburg State Medical University, Orenburg, RussiaNational Research Center for Preventive Medicine, Moscow, Russia1512201596690591128032016Copyright © 2015, Ryabchenko A.Y., Dolgov A.M., Denisov E.N., Russanova N.R., Gumanova N.G.2015<p><strong>Aim.</strong> To study features of tissue oxygenation and changes in nitroxidergic and endothelinergic mechanisms of circulation regulation in ischemic stroke and hypoxia modeling.</p>
<p><strong>Methods.</strong> 74 men and women with ischemic stroke were examined and experimental study on 30 outbred white male rats was conducted. All patients underwent computed tomography of the brain, neurological status was assessed and general clinical examination was performed. Tissue oxygen tension was measured percutaneuosly using polarographic method. Chronic hypoxic hypoxia was simulated in animals by daily 40-50 minutes inhalation of 10% oxygen mixture for 4 weeks. Blood oxygen tension was evaluated in patients and laboratory animals. Nitric oxide level of was assessed by determining its stable metabolites. Endothelin-1 level was determined by ELISA. Data was analyzed using «Statistica 8.0» software using non-parametric tests. Differences were considered as statistically significant at p <0.05.</p>
<p><strong>Results.</strong> Disturbances in endothelium-dependent mechanisms of vessels regulation, which were interrelated with oxygen tension changes were registered in patients with ischemic stroke. In laboratory animals amid the chronic hypoxic hypoxia concentration of the nitric oxide and endotheline-1 stabile metabolites increased.</p>
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