Effects of Different Fat-Carbohydrate Dietary Compositions and Probiotic Correction on the Severity of Myocardial Ischemia-Reperfusion Injury and Behavioral Responses in Rats with Systemic Inflammation

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Аннотация

The aim of this study was to compare the effects of intragastric administration of saturated and unsaturated fats of vegetable and animal origin for 37 days on behavioral characteristics in rats with a systemic inflammatory response (SVR). In parallel, the severity of ischemic-reperfusion injury (IRF) of the myocardium was evaluated using the global ischemia-reperfusion model of an isolated heart, as well as the mass fraction of fat in the brain and the levels of a number of cytokines and biologically active substances in blood plasma. The effects of a fat-carbohydrate diet of different composition on experimental endpoints were compared with the effect of probiotic therapy. The animals were assigned to one of 7 groups (n = 8 in each group): 1) control; 2) chemically induced colitis on the 30th day of the experiment and three-day administration of antimicrobial drugs; 3) probiotic correction: during the last 8 days of the experiment, a mixture of probiotic strains of Lactobacillus acidophilus and Bifidobacterium animalis subsp. lactis was administered; 4) sunflower oil and sucrose; 5) ghee and sucrose; 6) dehydrated margarine and sucrose; 7) ghee + sucrose. The systemic inflammatory response that occurs in chemically induced colitis in combination with the transient use of antimicrobial drugs was characterized by an increase in the number of white blood cells and an increase in the level of proinflammatory cytokines in the blood, as well as a decrease in the mass fraction of fat in the brain. These changes reduced the resistance of the myocardium to ischemic reperfusion injury, but the preventive administration of various fat-carbohydrate compositions had no effect on this process. On the other hand, the systemic inflammatory response was accompanied by significant impairments of conditioned reflex activity, exploratory behavior, and coordination and motor function, whereas probiotic therapy and a fat-carbohydrate diet of different composition partially correct these impairments. The most pronounced effect on the selected experimental endpoints was exerted by the fat-carbohydrate diet, in which the fat component was represented by hydrogenated vegetable fat.

Толық мәтін

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Авторлар туралы

Yu. Borshchev

Almazov National Medical Research Centre; Federal State Budgetary Institution “National Medical Research Center of Oncology named after N.N. Petrov” of the Ministry of Health of the Russian Federation

Хат алмасуға жауапты Автор.
Email: borshchev_yuyu@almazovcentre.ru
Ресей, Saint Petersburg; Saint Petersburg

I. Zubkov

Almazov National Medical Research Centre

Email: borshchev_yuyu@almazovcentre.ru
Ресей, Saint Petersburg

S. Minasyan

Almazov National Medical Research Centre; Pavlov First Saint Petersburg State Medical University

Email: borshchev_yuyu@almazovcentre.ru
Ресей, Saint Petersburg; Saint Petersburg

I. Burovenko

Almazov National Medical Research Centre

Email: borshchev_yuyu@almazovcentre.ru
Ресей, Saint Petersburg

V. Borshchev

Pavlov First Saint Petersburg State Medical University

Email: borshchev_yuyu@almazovcentre.ru
Ресей, Saint Petersburg

O. Borshcheva

Almazov National Medical Research Centre

Email: borshchev_yuyu@almazovcentre.ru
Ресей, Saint Petersburg

D. Burovenko

Moscow Institute of Physics and Technology

Email: borshchev_yuyu@almazovcentre.ru
Ресей, Moscow

G. Shul'meyster

Almazov National Medical Research Centre

Email: borshchev_yuyu@almazovcentre.ru
Ресей, Saint Petersburg

D. Korolev

Almazov National Medical Research Centre

Email: borshchev_yuyu@almazovcentre.ru
Ресей, Saint Petersburg

M. Galagudza

Almazov National Medical Research Centre; Pavlov First Saint Petersburg State Medical University; The Institute for Analytical Instrumentation

Email: borshchev_yuyu@almazovcentre.ru
Ресей, Saint Petersburg; Saint Petersburg; Saint Petersburg

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2. Fig. 1. Experimental design. AMC – antimicrobial mixture, i.g. – intragastric; CTR – control, PRC – probiotic therapy, DMS – anhydrous margarine and sucrose, SOS – sunflower oil and sucrose, SIR – systemic inflammatory response, CBS – ghee and sucrose, PFS – ghee and sucrose.

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3. Fig. 2. Effect of fat-carbohydrate diets of different compositions and probiotic strains on rat body weight, amount of adipose tissue, number of leukocytes and size of necrotic zone. (a) – Dynamics of animal body weight; (b) – mass coefficient of adipose tissue deposits; (c) – mass fraction of fat in the brain; (d) – number of leukocytes in the blood; (e) – size of necrotic zone. * – p < 0.05, in comparison with the KTR group; # – p < 0.05, in comparison with the SVO group in pairwise comparison with correction. See Fig. 1 for group designations.

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