CHANGES IN REPRODUCTIVE POTENTIAL, SURVIVAL AND METABOLIC ACTIVITY IN TWO SUCCESSIVE GENERATIONS OF DAPHNIA MAGNA IN THE PIPERONYL BUTOXIDE MEDIUM

Мұқаба

Дәйексөз келтіру

Толық мәтін

Аннотация

Changes in survival, fertility and metabolic activity in two generations of crustacean Daphnia magna in the piperonyl butoxide medium (PBO) were analyzed in concentrations of 50 to 800 ug/ml in a 21 day experiment in each generation. Metabolism disorders were evaluated using the MTT-assay traditionally applied in vitro to analyze cytotoxicity of medications. The MTT-assay shows in an integral way the amount of oxygen active forms, inactivation of mitochondrial oxidases, ratio of living and dead cells and function of antioxidant enzymes. It was found out that in the first generation, the PBO concentration of 353 ug/l is semi-lethal to Daphnia fertility and to the Daphnia survival it is 650 ug/l. In the second generation the toxic concentration is two-fold lower and amounts to 194 ug/l to fertility and to 200 ug/l to survival. It was revealed that PBO poses the cytotoxic effect on Daphnia.

Авторлар туралы

N. Kolesnikova

Obninsk Institute of Nuclear Power Engineering

Хат алмасуға жауапты Автор.
Email: Natasha442707245@mail.ru
249040 Obninsk, Kaluga region Ресей

A. Morozova

Obninsk Institute of Nuclear Power Engineering

Email: Sashkaa95@yandex.ru
249040 Obninsk, Kaluga region Ресей

D. Uskalova

Obninsk Institute of Nuclear Power Engineering

Email: uskalovad@mail.ru
249040 Obninsk, Kaluga region Ресей

E. Sarapultseva

Obninsk Institute of Nuclear Power Engineering;
National Research Nuclear University

Email: helen-bio@yandex.ru

249040 Obninsk, Kaluga region;

115409 Moscow

Ресей

Әдебиет тізімі

  1. Ryl’nikov V.A. Upravlenie chislennost’yu problemnyh vidov: uchebnoe posobie [Numerical management of problematic species: Tutorial] / V.A. Ryl’nicov, А.N. Matrosov, А.А. Kuznecov, А.А. Yakovlev, Н.В. Babich, А.А. Sludski, М.А. Tarasov, Ю.В. Toshigin, А.Ф.А. Kadirov. – Moskva: Institut pest-menedzmenta, 2012. V. 2. 169 p. (in Russian).
  2. Registry of Toxic Effects of Chemical Substances (RTECS). Comprehensive guide to the RTECS. Doris V. Sweet Ed. U.S. Department of health and human services, 1997. Public Health Service Centers for Disease Control and Prevention National Institute for Occupational Safety and Health Cincinnati, Ohio 45226.
  3. Onishenko G.G. Osnova ocenki riska dlya zdoroviya naseleniya pri vozdejstvii himicheskih veshestv, zagryaznyaushih sredu [Base on risk assessment to human health when exposed to environmental chemicals pollution] / G.G. Onishenko and U.A. Rakhmanin, ed. / G.G. Onishenko, S.M. Novikov, U.A. Rakhmanin, S.L. Avaliani, K.A. Bushtaev. − Мoscow: Scientific-Research Institute of Human Ecology and Environmental Hygiene them. AN Sysina, 2002 − 408 p. (in Russian).
  4. Organisation for Economic Co-operation and Development, 2012. Test No. 211. Daphnia magna Reproduction Test. OECD Guideline for Testing of Chemicals, Paris, France. doi: 10.1787/20745761.
  5. Cancer Cell Culture. Methods and Protocols. / I.A. Cree, ed. – Springer New York Dordrecht Heidelberg London: Human Press, 2011. P. 237−244.
  6. Sarapultseva E.I. Pryamye i otdalennye effect radiacionnogo oblucheniya prosteishih i rakoobraznyh. Dissertaciya doctora biologicheskih nauk [Direct and long-term effects of radiation exposure in protozoa and crustaceans. Dr. of. Sciences dissertation]. – Obninsk: Institute of Nuclear Power Engineering NRNU MEPhI, 2015. – 218 p. (in Russian).
  7. RD 64-085-89. MU “Metodicheskie osnovy biotestirovaniya i opredeleniya geneticheskoj opasnosti othodov, postupayushih v okruzaushyuyu sredu” [Methodical bases of biological testing and to determine the genetic dangers of waste going into the environment]. – Мoscow: Ministry of Medical Industry, 1990. 56 p. (in Russian).
  8. Vardavas A.I, Fragkiadaki P, Alegakis AK, Kouretas D, Goutzourelas N, Tsiaoussis J, Tsitsimpikou C, Stivaktakis P.D., Carvalho F, Tsatsakis A.M. Downgrading the systemic condition of rabbits after long term exposure to cypermethrin and piperonyl butoxide // Life Sci., 2016. 145. Р. 114-120. doi: 10.1016/j. lfs.2015.12.026.
  9. Cox C. Piperonyl Butoxide // Journal of pesticide reform, 2002. V. 22, NO. 2. P. 12 – 20.
  10. The WHO Recommended Classification of Pesticides by Hazard and Guidelines to Classification: 2009. IPCS. WHO Press, Geneva, 2010.
  11. U.S. Dept. of Agriculture. Agricultural Marketing Service. Science and Technology Division. 2001. Pesticide data program: Annual summary calendar year 2000. Appendix E. p. 39.
  12. Hayashi S, Ttaketa Y, OInoue K, Takahashi M, Matsuo S, Irie K, Watanabe G, Yoshida M. Effects of pyperonyl butoxide on the female reproductive tract in rats // J Toxicol Sci., 2013. 38 (6). Р. 891-902.
  13. Üner N, Piner P, Temiz Ö. Piperonyl butoxide increases oxidative toxicity of fenthion in the brain of Oreochromis niloticus // J Biochem Mol Toxicol., 2014. 28 (2). Р. 84-90. doi: 10.1002/jbt.21539.
  14. Chan H.H., Wajidi M.F., Zairi J. Molecular cloning and xenobiotic induction of seven novel cytochrome P450 monooxygenases in Aedes albopictus // J Insect Sci., 2014. 14:163. doi: 10.1093/jisesa/ieu025.

Қосымша файлдар

Қосымша файлдар
Әрекет
1. JATS XML
Жүктеу

© Kolesnikova N.I., Morozova A.O., Uskalova D.V., Sarapultseva E.I., 2017


СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: серия ПИ № ФС 77 - 81728 от 11 декабря 2013.