Email this article Apoptosis markers are molecular targets of sulfure mustard toxic action
- Authors: Rogovskaya N.Y.1, Babakov V.N.1
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Affiliations:
- Research Institute of Hygiene, Occupational Pathology and Human Ecology, Federal Medical Biological Agency
- Issue: No 5 (2017)
- Pages: 28-34
- Section: Articles
- Published: 28.10.2017
- URL: https://kazanmedjournal.ru/0869-7922/article/view/641036
- DOI: https://doi.org/10.36946/0869-7922-2017-5-28-34
- ID: 641036
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Abstract
Cellular in vitro test systems, which allow to determine apoptosis activation at different concentrations of sulfur mustard, were proposed. The efficiency of these test systems was evaluated by the action of N-acetylcysteine. As indicators of toxic effect, were evaluated an integral cytotoxicity and activation of the following targets: poly (ADP-ribose) polymerase (PARP1), caspase-3, caspase-9, transcription factor p53 and other markers of apoptosis in human SH-SY5Y cells extracts after sulfur mustard and sulfur mustard with N-acetylcysteine exposure. We can distinguished the following main target of sulfur mustard toxic action on the cells: caspase-3, caspase-9, PARP1 and p53 transcription factor, which in this system was the primary target and was activated after 6 h toxicant exposure. The active forms of enzymes caspase-3, caspase-9 and PARP1 significantly accumulate in cells after 24 h toxicant exposure.
About the authors
N. Yu. Rogovskaya
Research Institute of Hygiene, Occupational Pathology and Human Ecology, Federal Medical Biological Agency
Author for correspondence.
Email: noemail@neicon.ru
Russian Federation
V. N. Babakov
Research Institute of Hygiene, Occupational Pathology and Human Ecology, Federal Medical Biological Agency
Email: noemail@neicon.ru
Russian Federation
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