Inactivation of the gene responsible for the synthesis of gluconic acid in the genome of Pantoea brenneri

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Abstract

Abstract. To study the contribution of the glucose dehydrogenase (gcd) gene product to the development of ISR priming in plants, a strain of Pantoea brenneri 3.2 with a deletion of the gcd gene was obtained. Using the Lambda Red (λ Red) phage recombination system, we obtained a marker-free mutant strain of P. brenneri 3.2 Δgcd. Inactivation of the gcd glucose dehydrogenase gene resulted in a 2.5-fold decrease in the strain’s ability to solubilize tricalcium phosphate on the solid nutrient medium NBRIP.

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About the authors

A. D. Suleimanova

Kazan (Volga Region) Federal University

Author for correspondence.
Email: Aliya.kzn@gmail.com
Russian Federation, Kazan, 420008

L. V. Sokolnikova

Kazan (Volga Region) Federal University

Email: Aliya.kzn@gmail.com
Russian Federation, Kazan, 420008

D. S. Bulmakova

Kazan (Volga Region) Federal University

Email: Aliya.kzn@gmail.com
Russian Federation, Kazan, 420008

E. A. Egorova

Kazan (Volga Region) Federal University

Email: Aliya.kzn@gmail.com
Russian Federation, Kazan, 420008

M. R. Sharipova

Kazan (Volga Region) Federal University

Email: Aliya.kzn@gmail.com
Russian Federation, Kazan, 420008

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2. Fig. 1. Electrophoresis of PCR products at the stages of obtaining the mutant strain P. brenneri 3.2 Δgcd

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3. Fig. 2. a – Growth of the native strain P. brenneri 3.2 and its mutant for the gene involved in the synthesis of gluconic acid on LB medium. *P < 0.05, **P < 0.01, ***P < 0.001; b – ability of the native and mutant strains to solubilize tricalcium phosphate.

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